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Literature summary for 1.4.1.13 extracted from
- Expression and functional analysis of glutamate synthase small subunit-like proteins from archaeon Pyrococcus horikoshii (2011), Microbiol. Res., 166, 294-303.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PH0876, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain Rosetta (DE3) | Pyrococcus horikoshii |
| gene PH1873, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain Rosetta (DE3) | Pyrococcus horikoshii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetic analysis, overview | Pyrococcus horikoshii | |
| 0.042 | - |
NADPH | pH 7.5, 37°C, pGLTY2 | Pyrococcus horikoshii |  |
| 0.11 | - |
NADPH | pH 7.5, 70°C, pGLTY2 | Pyrococcus horikoshii | |
| 6.42 | - |
NADPH | pH 7.5, 37°C, pGLTZ | Pyrococcus horikoshii | |
| 16.3 | - |
NADPH | pH 7.5, 70°C, pGLTZ | Pyrococcus horikoshii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51900 | - |
x * 51900, pGLTY2, SDS-PAGE, x * 52000, pGLTY1, SDS-PAGE | Pyrococcus horikoshii |
| 52000 | - |
x * 51900, pGLTY2, SDS-PAGE, x * 52000, pGLTY1, SDS-PAGE | Pyrococcus horikoshii |
| 52400 | - |
x * 52400, SDS-PAGE | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Pyrococcus horikoshii | - |
NH3 + 2-oxoglutarate + NADPH + H+ | - |
? | |
| NH3 + 2-oxoglutarate + NADPH + H+ | Pyrococcus horikoshii | - |
L-glutamate + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | A0A171 | orf PH0876 | - |
| Pyrococcus horikoshii | B3FYT6 | orf PH1873 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | - |
Pyrococcus horikoshii | NH3 + 2-oxoglutarate + NADPH + H+ | - |
? | |
| additional information | assay with artificial eelectron acceptor iodonitrotetrazolium chloride | Pyrococcus horikoshii | ? | - |
? | |
| NH3 + 2-oxoglutarate + NADPH + H+ | - |
Pyrococcus horikoshii | L-glutamate + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51900, pGLTY2, SDS-PAGE, x * 52000, pGLTY1, SDS-PAGE | Pyrococcus horikoshii |
| ? | x * 52400, SDS-PAGE | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GOGAT | - |
Pyrococcus horikoshii |
| pGLTY1 | - |
Pyrococcus horikoshii |
| pGLTY2 | - |
Pyrococcus horikoshii |
| pGLTZ | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pyrococcus horikoshii |
| 70 | - |
- |
Pyrococcus horikoshii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 70 | - |
Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 100 | purified enzyme, 10 min, stable up to 90°C | Pyrococcus horikoshii |
| 100 | - |
purified enzyme, 10 min, loss of activity | Pyrococcus horikoshii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.28 | - |
NADPH | pH 7.5, 37°C, pGLTY2 | Pyrococcus horikoshii | |
| 6.42 | - |
NADPH | pH 7.5, 37°C, pGLTZ | Pyrococcus horikoshii | |
| 16.3 | - |
NADPH | pH 7.5, 70°C, pGLTZ | Pyrococcus horikoshii | |
| 26.8 | - |
NADPH | pH 7.5, 70°C, pGLTY2 | Pyrococcus horikoshii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pyrococcus horikoshii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Pyrococcus horikoshii | |
| NADPH | - |
Pyrococcus horikoshii | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetic and molecular evolutionary analyses | Pyrococcus horikoshii |
| physiological function | key enzyme in the early stages of ammonia assimilation in bacteria, algae and plants, catalyzing the reductive transamidation of the amido nitrogen from glutamine to 2-oxoglutarate to form two molecules of glutamate | Pyrococcus horikoshii |
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