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Literature summary for 1.4.1.12 extracted from

  • Fukuyama, S.; Mihara, H.; Miyake, R.; Ueda, M.; Esaki, N.; Kurihara, T.
    Characterization of a thermostable 2,4-diaminopentanoate dehydrogenase from Fervidobacterium nodosum Rt17-B1 (2014), J. Biosci. Bioeng., 117, 551-556 .
    View publication on PubMed
Search for more literature for 1.4.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Fervidobacterium nodosum

Inhibitors

Inhibitors Comment Organism Structure
(2R,4S)-2,4-diaminopentanoate uncompetitive substrate inhibition Fervidobacterium nodosum
Ca2+ 85% residual activity at 0.5 mM Fervidobacterium nodosum
Co2+ 34% residual activity at 0.5 mM Fervidobacterium nodosum
Cu2+ complete inhibition at 0.5 mM Fervidobacterium nodosum
D-ornithine uncompetitive inhibition Fervidobacterium nodosum
Fe2+ 84% residual activity at 0.5 mM Fervidobacterium nodosum
Mg2+ 65% residual activity at 0.5 mM Fervidobacterium nodosum
additional information not inhibited by EDTA, KBr, KCl, KNO3, CH3COOK, and K2SO4 Fervidobacterium nodosum
Ni2+ 2% residual activity at 0.5 mM Fervidobacterium nodosum
Zn2+ 2% residual activity at 0.5 mM Fervidobacterium nodosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 NAD+ at pH 8.5 and 55°C Fervidobacterium nodosum
0.2
-
 (2R,4S)-2,4-diaminopentanoate at pH 8.5 and 55°C Fervidobacterium nodosum
2
-
 NADP+ at pH 8.5 and 55°C Fervidobacterium nodosum
2
-
 (2R,5S)-2,5-diaminohexanoate at pH 8.5 and 55°C Fervidobacterium nodosum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mn2+ has no significant effect on enzyme activity Fervidobacterium nodosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,4S)-2,4-diaminopentanoate + H2O + NAD+ Fervidobacterium nodosum
-
 (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+
-
 ?
(2R,4S)-2,4-diaminopentanoate + H2O + NADP+ Fervidobacterium nodosum
-
 (2R)-2-amino-4-oxopentanoate + NH3 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Fervidobacterium nodosum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,4S)-2,4-diaminopentanoate + H2O + NAD+
-
 Fervidobacterium nodosum (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+
-
 ?
(2R,4S)-2,4-diaminopentanoate + H2O + NADP+
-
 Fervidobacterium nodosum (2R)-2-amino-4-oxopentanoate + NH3 + NADPH + H+
-
 ?
(2R,5S)-2,5-diaminohexanoate + H2O + NADP+
-
 Fervidobacterium nodosum (2R)-2-amino-5-oxohexanoate + NH3 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Fervidobacterium nodosum

Synonyms

Synonyms Comment Organism
2,4-DAPDH
-
 Fervidobacterium nodosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Fervidobacterium nodosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 the half-denaturation time at 90°C is 38 min and 2 min at 100°C Fervidobacterium nodosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0006
-
 (2R,5S)-2,5-diaminohexanoate at pH 8.5 and 55°C Fervidobacterium nodosum
57
-
 NADP+ at pH 8.5 and 55°C Fervidobacterium nodosum
65
-
 NAD+ at pH 8.5 and 55°C Fervidobacterium nodosum
65
-
 (2R,4S)-2,4-diaminopentanoate at pH 8.5 and 55°C Fervidobacterium nodosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Fervidobacterium nodosum

pH Range

pH Minimum pH Maximum Comment Organism
9 10 the enzyme shows more than 70% of maximum activity at pH 9.0-10.0 Fervidobacterium nodosum

Cofactor

Cofactor Comment Organism Structure
NAD+ the activity of the enzyme with NAD+ is 5.4times higher than that with NADP+ Fervidobacterium nodosum
NADP+ the activity of the enzyme with NAD+ is 5.4times higher than that with NADP+ Fervidobacterium nodosum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 D-ornithine at pH 8.5 and 55°C Fervidobacterium nodosum

General Information

General Information Comment Organism
metabolism the enzyme plays a regulatory role in the oxidative ornithine degradation pathway Fervidobacterium nodosum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
29
-
 NADP+ at pH 8.5 and 55°C Fervidobacterium nodosum
330
-
 (2R,4S)-2,4-diaminopentanoate at pH 8.5 and 55°C Fervidobacterium nodosum
1300
-
 NAD+ at pH 8.5 and 55°C Fervidobacterium nodosum