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Literature summary for 1.4.1.1 extracted from
- Purification and characterization of alanine dehydrogenase from Streptomyces anulatus for its application as a bioreceptor in biosensor (2018), Process Biochem., 68, 73-82 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Streptomyces anulatus |  |
| DTT | - |
Streptomyces anulatus | |
| EDTA | - |
Streptomyces anulatus | |
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | alanine dehydrogenase from Streptomyces anulatus can be used as a bioreceptor in an ammonium biosensor that can detect ammonium ions in water samples. The sensor shows linear response in the range of 0.1-300mM NH4+ with the detection limit of 0.01 mM NH4+ and response time of 20 s. The sensor is showing good response at wide pH range (pH 5-11) and temperature range (20-50°C) suggesting its usage at ambient and non-ambient conditions. The sensor is successfully validated with Nessler's reagent method by using different water samples | Streptomyces anulatus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an amperometric biosensor using AlaDH of Streptomyces anulatus amidst the working electrode (3 mm diameter) present at the center of the screen printed electrode. The mixture contains AlaDH (80 mg/ml of freeze dried enzyme), NADH (200 mg/ml), pyruvate (80 mg/ml) and the binder 1% poly HEMA (poly-hydroxyethylmethacrylate) in 50 mM Tris-HCl buffer, pH 8.5. The mixture is allowed to dry at 4°C. The weight of the sensor strip after immobilizing the mixture is 0.56 g sensor strips are incubated at different temperatures (10-70°C) for 5 min before placing the samples (10 mM ammonium chloride dissolved in buffers of different pH) on the strip during the measurement of output current. The output current is measured by cyclic voltammetry by using a potentiostat | Streptomyces anulatus |
General Stability
| General Stability | Organism |
|---|---|
| the purified enzyme is stabilized by EDTA, 2-mercaptoethanol, and DTT with effectivity in increasing order | Streptomyces anulatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Streptomyces anulatus | |
| D-alanine | AlaDH enzyme activity is completely inhibited by D-alanine in a competitive manner | Streptomyces anulatus | |
| Fe2+ | - |
Streptomyces anulatus | |
| HgCl2 | complete inhibition | Streptomyces anulatus | |
| Mn2+ | - |
Streptomyces anulatus | |
| additional information | no inhibition by L-serine and L-valine | Streptomyces anulatus | |
| Zn2+ | - |
Streptomyces anulatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.064 | - |
NADH | pH 8.5, 40°C | Streptomyces anulatus | |
| 0.126 | - |
NAD+ | pH 10.0, 40°C | Streptomyces anulatus | |
| 0.613 | - |
pyruvate | pH 8.5, 40°C | Streptomyces anulatus | |
| 4.79 | - |
L-alanine | pH 10.0, 40°C | Streptomyces anulatus | |
| 32.82 | - |
NH3 | pH 8.5, 40°C | Streptomyces anulatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Streptomyces anulatus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine + H2O + NAD+ | Streptomyces anulatus | - |
pyruvate + NH3 + NADH + H+ | - |
r | |
| L-alanine + H2O + NAD+ | Streptomyces anulatus MTCC 2528 | - |
pyruvate + NH3 + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces anulatus | A0A6G3T2F2 | - |
- |
| Streptomyces anulatus MTCC 2528 | A0A6G3T2F2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ammonium sulfate fractionation, dialysis, anion exchange chromatography, again dialysis, and gel filtration | Streptomyces anulatus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified native enzyme, significant loss in enzyme activity at 25°C and 35°C on the 15th day and almost no activity on the 120th day, but the enzyme retains most of the activity in the presence of EDTA, 2-ME and DTT | Streptomyces anulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine + H2O + NAD+ | - |
Streptomyces anulatus | pyruvate + NH3 + NADH + H+ | - |
r | |
| L-alanine + H2O + NAD+ | - |
Streptomyces anulatus MTCC 2528 | pyruvate + NH3 + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 61000, SDS-PAGE | Streptomyces anulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALADH | - |
Streptomyces anulatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Streptomyces anulatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | activity range | Streptomyces anulatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the enzyme retains more than 85% of its original activity after incubation at 60°C for 30 min in the presence of DTT | Streptomyces anulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
reductive amination | Streptomyces anulatus |
| 10 | - |
oxidative deamination | Streptomyces anulatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | activity range | Streptomyces anulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Streptomyces anulatus | |
| NADH | - |
Streptomyces anulatus | |
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