Cloned (Comment) | Organism |
---|---|
gene ald, quantitative and qualitative RT-PCR enzyme expression analysis | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a DELTAaldR mutant strain | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | Mycolicibacterium smegmatis | - |
pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | Mycolicibacterium smegmatis ATCC 700084 | - |
pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | Mycolicibacterium smegmatis mc(2)155 | - |
pyruvate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0QVQ8 | Mycobacterium smegmatis | - |
Mycolicibacterium smegmatis ATCC 700084 | A0QVQ8 | Mycobacterium smegmatis | - |
Mycolicibacterium smegmatis mc(2)155 | A0QVQ8 | Mycobacterium smegmatis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | - |
Mycolicibacterium smegmatis | pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | - |
Mycolicibacterium smegmatis ATCC 700084 | pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | - |
Mycolicibacterium smegmatis mc(2)155 | pyruvate + NH3 + NADH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.2 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycolicibacterium smegmatis |
Organism | Comment | Expression |
---|---|---|
Mycolicibacterium smegmatis | expression of the ald gene is strongly upregulated in Mycolicibacterium smegmatis grown in the presence of alanine. Alanine-dependent regulation of ald is mediated by the AldR transcriptional regulator that belongs to the Lrp/AsnC (leucine-responsive regulatory protein/asparagine synthase C) family. A decrease in functionality of the ETC shifts the redox state of the NADH/NAD+x02 pool toward a more reduced state, which in turn leads to an increase in cellular levels of alanine by Ald catalyzing the conversion of pyruvate to alanine with the concomitant oxidation of NADH to NAD+. The induction of ald expression under respiration-inhibitory conditions in Mycolicibacterium smegmatis is mediated by the alanine-responsive AldR transcriptional regulator. Respiration inhibition by KCN induces ald expression in the wild-type strain, but not DELTAaldR mutant strain of Mycolicibacterium smegmatis grown under aerobic conditions | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the inhibition of electron flux through the respiratory electron transport chain (ETC) by either the disruption of the gene for the major terminal oxidase (aa3 cytochrome c oxidase) or treatment with KCN results in the induction of ald encoding alanine dehydrogenase in Mycolicibacterium smegmatis. A decrease in functionality of the ETC shifts the redox state of the NADH/NAD+x02pool toward a more reduced state, which in turn leads to an increase in cellular levels of alanine by Ald catalyzing the conversion of pyruvate to alanine with the concomitant oxidation of NADH to NAD+. The induction of ald expression under respiration-inhibitory conditions in Mycolicibacterium smegmatis is mediated by the alanine-responsive AldR transcriptional regulator. The growth defect of the bacteria by respiration inhibition is exacerbated by inactivation of the ald gene | Mycolicibacterium smegmatis |
metabolism | expression of the ald gene is strongly upregulated in Mycolicibacterium smegmatis grown in the presence of alanine. Alanine-dependent regulation of ald is mediated by the AldR transcriptional regulator that belongs to the Lrp/AsnC (leucine-responsive regulatory protein/asparagine synthase C) family | Mycolicibacterium smegmatis |
physiological function | Ald is beneficial to Mycolicibacterium smegmatis in its adaptation and survival under respiration-inhibitory conditions by maintaining NADH/NAD+ homeostasis. Ald is required for optimal mycobacterial growth under severe respiration-inhibitory conditions | Mycolicibacterium smegmatis |