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Literature summary for 1.4.1.1 extracted from

  • Jeong, J.A.; Park, S.W.; Yoon, D.; Kim, S.; Kang, H.Y.; Oh, J.I.
    Roles of alanine dehydrogenase and induction of its gene in Mycobacterium smegmatis under respiration-inhibitory conditions (2018), J. Bacteriol., 200, e00152-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ald, quantitative and qualitative RT-PCR enzyme expression analysis Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
additional information generation of a DELTAaldR mutant strain Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + H2O + NAD+ Mycolicibacterium smegmatis
-
pyruvate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+ Mycolicibacterium smegmatis ATCC 700084
-
pyruvate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+ Mycolicibacterium smegmatis mc(2)155
-
pyruvate + NH3 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0QVQ8 Mycobacterium smegmatis
-
Mycolicibacterium smegmatis ATCC 700084 A0QVQ8 Mycobacterium smegmatis
-
Mycolicibacterium smegmatis mc(2)155 A0QVQ8 Mycobacterium smegmatis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + H2O + NAD+
-
Mycolicibacterium smegmatis pyruvate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+
-
Mycolicibacterium smegmatis ATCC 700084 pyruvate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+
-
Mycolicibacterium smegmatis mc(2)155 pyruvate + NH3 + NADH + H+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.2
-
assay at Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mycolicibacterium smegmatis

Expression

Organism Comment Expression
Mycolicibacterium smegmatis expression of the ald gene is strongly upregulated in Mycolicibacterium smegmatis grown in the presence of alanine. Alanine-dependent regulation of ald is mediated by the AldR transcriptional regulator that belongs to the Lrp/AsnC (leucine-responsive regulatory protein/asparagine synthase C) family. A decrease in functionality of the ETC shifts the redox state of the NADH/NAD+x02 pool toward a more reduced state, which in turn leads to an increase in cellular levels of alanine by Ald catalyzing the conversion of pyruvate to alanine with the concomitant oxidation of NADH to NAD+. The induction of ald expression under respiration-inhibitory conditions in Mycolicibacterium smegmatis is mediated by the alanine-responsive AldR transcriptional regulator. Respiration inhibition by KCN induces ald expression in the wild-type strain, but not DELTAaldR mutant strain of Mycolicibacterium smegmatis grown under aerobic conditions up

General Information

General Information Comment Organism
malfunction the inhibition of electron flux through the respiratory electron transport chain (ETC) by either the disruption of the gene for the major terminal oxidase (aa3 cytochrome c oxidase) or treatment with KCN results in the induction of ald encoding alanine dehydrogenase in Mycolicibacterium smegmatis. A decrease in functionality of the ETC shifts the redox state of the NADH/NAD+x02pool toward a more reduced state, which in turn leads to an increase in cellular levels of alanine by Ald catalyzing the conversion of pyruvate to alanine with the concomitant oxidation of NADH to NAD+. The induction of ald expression under respiration-inhibitory conditions in Mycolicibacterium smegmatis is mediated by the alanine-responsive AldR transcriptional regulator. The growth defect of the bacteria by respiration inhibition is exacerbated by inactivation of the ald gene Mycolicibacterium smegmatis
metabolism expression of the ald gene is strongly upregulated in Mycolicibacterium smegmatis grown in the presence of alanine. Alanine-dependent regulation of ald is mediated by the AldR transcriptional regulator that belongs to the Lrp/AsnC (leucine-responsive regulatory protein/asparagine synthase C) family Mycolicibacterium smegmatis
physiological function Ald is beneficial to Mycolicibacterium smegmatis in its adaptation and survival under respiration-inhibitory conditions by maintaining NADH/NAD+ homeostasis. Ald is required for optimal mycobacterial growth under severe respiration-inhibitory conditions Mycolicibacterium smegmatis