Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation | Rhodobacter capsulatus | |
Cys | activates | Rhodobacter capsulatus | |
dithioerythritol | activates | Rhodobacter capsulatus | |
dithiothreitol | activation | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Ala | 5 mM, 34% inhibition | Rhodobacter capsulatus | |
L-Ser | 10 mM, 26% inhibition; inhibition of aminating activity | Rhodobacter capsulatus | |
p-hydroxymercuribenzoate | no effect on aminating activity, inhibition of deaminating activity | Rhodobacter capsulatus | |
pyruvate | product inhibition | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
pyruvate | - |
Rhodobacter capsulatus | |
0.15 | - |
NAD+ | - |
Rhodobacter capsulatus | |
0.25 | - |
NADH | - |
Rhodobacter capsulatus | |
1.25 | - |
L-alanine | - |
Rhodobacter capsulatus | |
16 | - |
NH4+ | - |
Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
6 * 42000, SDS-PAGE | Rhodobacter capsulatus |
240000 | 241000 | gel filtration | Rhodobacter capsulatus |
246000 | - |
non-denaturing PAGE | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | Rhodobacter capsulatus | alternative route for ammonia assimilation when glutamine synthetase is inactivated | pyruvate + NH3 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Storage Stability | Organism |
---|---|
4°C, stable | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | - |
Rhodobacter capsulatus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | alternative route for ammonia assimilation when glutamine synthetase is inactivated | Rhodobacter capsulatus | pyruvate + NH3 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 42000, SDS-PAGE | Rhodobacter capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
deaminating activity | Rhodobacter capsulatus |
30 | - |
aminating activity | Rhodobacter capsulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
50% loss of aminating activity after 20 min, 50% loss of deaminating activity after 27 min | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
amination of pyruvate | Rhodobacter capsulatus |
10.5 | - |
deamination of L-Ala | Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for | Rhodobacter capsulatus | |
NADH | - |
Rhodobacter capsulatus | |
NADPH | aminating activity absolutely specific for | Rhodobacter capsulatus |