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Literature summary for 1.4.1.1 extracted from

  • Caballero, F.J.; Cardenas, J.; Castillo, F.
    Purification and properties of L-alanine dehydrogenase of the phototrophic bacterium Rhodobacter capsulatus E1F1 (1989), J. Bacteriol., 171, 3205-3210.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Rhodobacter capsulatus
Cys activates Rhodobacter capsulatus
dithioerythritol activates Rhodobacter capsulatus
dithiothreitol activation Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
L-Ala 5 mM, 34% inhibition Rhodobacter capsulatus
L-Ser 10 mM, 26% inhibition; inhibition of aminating activity Rhodobacter capsulatus
p-hydroxymercuribenzoate no effect on aminating activity, inhibition of deaminating activity Rhodobacter capsulatus
pyruvate product inhibition Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
pyruvate
-
Rhodobacter capsulatus
0.15
-
NAD+
-
Rhodobacter capsulatus
0.25
-
NADH
-
Rhodobacter capsulatus
1.25
-
L-alanine
-
Rhodobacter capsulatus
16
-
NH4+
-
Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
6 * 42000, SDS-PAGE Rhodobacter capsulatus
240000 241000 gel filtration Rhodobacter capsulatus
246000
-
non-denaturing PAGE Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Ala + H2O + NAD+ Rhodobacter capsulatus alternative route for ammonia assimilation when glutamine synthetase is inactivated pyruvate + NH3 + NADH
-
?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodobacter capsulatus

Storage Stability

Storage Stability Organism
4°C, stable Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
Rhodobacter capsulatus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+ alternative route for ammonia assimilation when glutamine synthetase is inactivated Rhodobacter capsulatus pyruvate + NH3 + NADH
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 42000, SDS-PAGE Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
deaminating activity Rhodobacter capsulatus
30
-
aminating activity Rhodobacter capsulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
50% loss of aminating activity after 20 min, 50% loss of deaminating activity after 27 min Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
amination of pyruvate Rhodobacter capsulatus
10.5
-
deamination of L-Ala Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Rhodobacter capsulatus
NADH
-
Rhodobacter capsulatus
NADPH aminating activity absolutely specific for Rhodobacter capsulatus