Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bathocuproin | - |
Pseudomonas putida | |
Cuprizone | - |
Pseudomonas putida | |
cyanide | not | Pseudomonas putida | |
additional information | not inhibitory: diethyldithiocarbamate, p-chloromercuribenzoate, arsenite, iodoacetamide, 2-n-heptyl-4-hydroxyquinoline N-oxide, azide, alpha,alpha'-bipyridyl, Tiron (i.e. disodium 1,2-dihydroxybenzene-3,5-disulfonate), 8-hydroxyquinoline, menadione | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | 0.05 | 2-Furoyl-CoA | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | - |
Pseudomonas putida | |
additional information | no Fe | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
3270000 | - |
sedimentation equilibrium | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-furoyl-CoA + H2O + acceptor | Pseudomonas putida | - |
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor | - |
? | |
2-furoyl-CoA + H2O + acceptor | Pseudomonas putida F2 | - |
S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida F2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor | mechanism | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-furoyl-CoA + H2O + acceptor | - |
Pseudomonas putida | S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor | - |
? | |
2-furoyl-CoA + H2O + acceptor | acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida | Pseudomonas putida | S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor | product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA | ? | |
2-furoyl-CoA + H2O + acceptor | - |
Pseudomonas putida F2 | S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor | - |
? | |
2-furoyl-CoA + H2O + acceptor | acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida | Pseudomonas putida F2 | S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor | product is tautomerized to 5-oxo-DELTA2-dihydro-2-furoyl-CoA | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | 2-furoyl-CoA + methylene blue | Pseudomonas putida |