Cloned (Comment) | Organism |
---|---|
identification of multiple copies of putative genes encoding the structural components of a class II BCR in sulfate reducing, Fe(III)-respiring and syntrophic bacteria | Desulfosarcina cetonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme shows Michaelis-Menten kinetics | Desulfosarcina cetonica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 185 per enzyme complex | Desulfosarcina cetonica | |
additional information | theoretical analyses show 4 W-pterins, selenocysteines, 6 flavin adenine dinucleotides, 4 Zn, and 48 Fe-S clusters per enzyme complex | Desulfosarcina cetonica | |
Tungsten | 4 W per enzyme complex, in the active site pterin cofactor | Desulfosarcina cetonica | |
Zn2+ | 3.4 per enzyme complex | Desulfosarcina cetonica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
950000 | - |
soluble Bam[(BC)2DEFGHI]2 complex | Desulfosarcina cetonica |
956000 | - |
amino acid sequence calculation | Desulfosarcina cetonica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | Desulfosarcina cetonica | high activity | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | Desulfosarcina cetonica DSMZ 7267 | high activity | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | Desulfosarcina cetonica 480 | high activity | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfosarcina cetonica | - |
- |
- |
Desulfosarcina cetonica 480 | - |
- |
- |
Desulfosarcina cetonica DSMZ 7267 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from Desulfosarcina cetonica cells grown on benzoate and sulfate 17.3fold by anion exchange chromatography and gel filtration | Desulfosarcina cetonica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate = benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | class II BCRs drive BzCoA reduction by flavin-based electron bifurcation (FBEB) | Desulfosarcina cetonica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity (reverse reaction), purified native enzyme, pH 6.0, temperature not specified in the publication | Desulfosarcina cetonica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | high activity | Desulfosarcina cetonica | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | high activity | Desulfosarcina cetonica DSMZ 7267 | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O | high activity | Desulfosarcina cetonica 480 | cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate | - |
? | |
additional information | class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation | Desulfosarcina cetonica | ? | - |
- |
|
additional information | class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation | Desulfosarcina cetonica DSMZ 7267 | ? | - |
- |
|
additional information | class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation | Desulfosarcina cetonica 480 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | the class II BCR complex is composed of the BamBCDEGHI subunits | Desulfosarcina cetonica |
Synonyms | Comment | Organism |
---|---|---|
1,5-dienoyl-CoA:methyl viologen oxidoreductase | - |
Desulfosarcina cetonica |
BCR | - |
Desulfosarcina cetonica |
class II BCR complex | - |
Desulfosarcina cetonica |
class II benzoyl-coenzyme A reductase complex | - |
Desulfosarcina cetonica |
DCO | - |
Desulfosarcina cetonica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Desulfosarcina cetonica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Desulfosarcina cetonica | |
FAD | 4.8 FAD per enzyme complex | Desulfosarcina cetonica | |
Fe-S center | 48 Fe-S clusters per enzyme complex. The BamC subunit harbours three electron-transferring [4Fe-4S] clusters | Desulfosarcina cetonica | |
tungsto-bispterin cofactor | - |
Desulfosarcina cetonica |
General Information | Comment | Organism |
---|---|---|
evolution | high-molecular class II BCR metalloenzyme machineries are remarkably conserved in strictly anaerobic bacteria with regard to subunit architecture and cofactor content, but their subcellular localization and electron acceptor preference may differ as a result of adaptations to variable energy metabolisms. There are two non-related classes of BCRs that follow fundamentally different strategies for BzCoA dearomatization. Class I BCRs couple electron transfer from a reduced ferredoxin or the aromatic ring to a stoichiometric hydrolysis of two MgATP. These homotetrameric, three [4Fe-4S] cluster containing enzymes are composed of an ATP-hydrolyzing module composed of two highly similar subunits and a heterodimeric BzCoA reducing module. Class I BCRs are abundant in facultatively anaerobic bacteria and have been isolated and characterized from aromatic compound degrading, denitrifying Thauera species. The ATP-independent class II BCRs occur in strictly anaerobic sulfate-, metal-oxide-reducing or syntrophic bacteria that gain far less energy during the oxidation of aromatics to CO2 or acetate. Class II BCR occur in the Fe(III)-respiring Geobacter metallireducens | Desulfosarcina cetonica |
metabolism | most monocyclic aromatic compounds including the BTEX (benzene, toluene, ethylbenzene and xylenes) are first converted in channelling enzymatic reaction sequences to the central intermediate benzoyl-coenzyme A (BzCoA), which then serves as substrate for dearomatizing cyclohexa-1,5-diene-1-carboxyl-CoA (1,5-dienoyl-CoA) forming BzCoA reductases (BCRs) | Desulfosarcina cetonica |
physiological function | benzoyl-CoA reductases (BCRs) catalyse a key reaction in the anaerobic degradation pathways of monocyclic aromatic substrates, the dearomatization of benzoyl-CoA (BzCoA) to cyclohexa-1,5-diene-1-carboxyl-CoA (1,5-dienoyl-CoA) at the negative redox potential limit of diffusible enzymatic substrate/product couples. The class II BCR complex composed of BamBCDEGHI subunits is supposed to drive endergonic benzene ring reduction at an active site W-pterin cofactor by flavin-based electron bifurcation | Desulfosarcina cetonica |