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Literature summary for 1.3.7.15 extracted from

  • Yamamoto, H.; Mizoguchi, T.; Tsukatani, Y.; Tamiaki, H.; Kurisu, G.; Fujita, Y.
    Chlorophyllide a oxidoreductase preferentially catalyzes 8-vinyl reduction over B-ring reduction of 8-vinyl chlorophyllide a in the late steps of bacteriochlorophyll biosynthesis (2020), ChemBioChem, 21, 1760-1766 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes bchX and bchY, recombinant expression of GST-tagged BchX and BchY in Escherichia coli Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
8-vinyl chlorophyllide a inhibitory effect of 8 V-Chlide on 3 V-BChlide production inhibitory effect of 8 V-Chlide on the C7=C8 reduction of COR Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
8-vinyl chlorophyllide a recombinant enzyme BchX/BchY, pH and temperature not specified in the publication Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ Rhodobacter capsulatus
-
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ Rhodobacter capsulatus NBRC 16581
-
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ Rhodobacter capsulatus ATCC BAA-309
-
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ Rhodobacter capsulatus SB1003
-
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Rhodobacter capsulatus
-
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Rhodobacter capsulatus NBRC 16581
-
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Rhodobacter capsulatus ATCC BAA-309
-
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Rhodobacter capsulatus SB1003
-
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus P26177 gene bchX
-
Rhodobacter capsulatus ATCC BAA-309 P26177 gene bchX
-
Rhodobacter capsulatus NBRC 16581 P26177 gene bchX
-
Rhodobacter capsulatus SB1003 P26177 gene bchX
-

Purification (Commentary)

Purification (Comment) Organism
copurification of recombinant GST-tagged BchX and BchY from Escherichia coli by glutathione affinity chromatography Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+
-
Rhodobacter capsulatus bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+
-
Rhodobacter capsulatus NBRC 16581 bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+
-
Rhodobacter capsulatus ATCC BAA-309 bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+
-
Rhodobacter capsulatus SB1003 bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Rhodobacter capsulatus chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Rhodobacter capsulatus NBRC 16581 chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Rhodobacter capsulatus ATCC BAA-309 chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Rhodobacter capsulatus SB1003 chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
additional information enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 Rhodobacter capsulatus ?
-
-
additional information enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 Rhodobacter capsulatus NBRC 16581 ?
-
-
additional information enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 Rhodobacter capsulatus ATCC BAA-309 ?
-
-
additional information enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 Rhodobacter capsulatus SB1003 ?
-
-

Subunits

Subunits Comment Organism
More In contrast to BciA and BciB, COR is a nitrogenase-like complex enzyme consisting of two separable components, the X protein (a BchX dimer) and the YZ protein (a BchY-BchZ heterotetramer). The X protein serves as the reductase component by transferring electrons from a [4Fe-4S] cluster held in the interface between the BchX homodimer to the YZ protein. The YZ protein is the catalytic component, as it accepts electrons from the X protein and transfers them to the Chlide substrate via a [4Fe-4S] cluster, to produce 3-vinyl bacteriochlorophyllide a (3 V-BChlide) Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
chlorophyllide a oxidoreductase
-
Rhodobacter capsulatus
COR
-
Rhodobacter capsulatus
COR 8VR
-
Rhodobacter capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00027
-
8-vinyl chlorophyllide a recombinant enzyme BchX/BchY, pH and temperature not specified in the publication Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Rhodobacter capsulatus
Fe-S center a [4Fe-4S] cluster is bound at the interface between the BchX homodimer and the YZ protein of the COR enzyme complex Rhodobacter capsulatus
Ferredoxin
-
Rhodobacter capsulatus

General Information

General Information Comment Organism
metabolism bacteriochlorophyll a (BChl) is an essential pigment for anoxygenic photosynthesis. In late steps of the BChl biosynthesis of Rhodobacter capsulatus, the C8 vinyl group and C7=C8 double bond of 8-vinyl chlorophyllide a (8 V-Chlide) are reduced by a C8 vinyl reductase (8VR), BciA (EC 1.3.1.75) or BciB (EC 1.3.7.13), and a nitrogenase-like enzyme, chlorophyllide a oxidoreductase (COR), respectively, to produce 3-vinyl-bacteriochlorphyllide a. BciB is a FAD-containing FeS protein that uses reduced ferredoxin as the reductant for the 8-vinyl reduction of 8 V-Chlide or 8 V-PChlide, while BciA utilizes NADPH Rhodobacter capsulatus
physiological function bacteriochlorophyll a (BChl) is an essential pigment for anoxygenic photosynthesis. In late steps of the BChl biosynthesis of Rhodobacter capsulatus, the C8 vinyl group and C7=C8 double bond of 8-vinyl chlorophyllide a (8 V-Chlide) are reduced by a C8 vinyl reductase (8VR), BciA (EC 1.3.1.75) or BciB (EC 1.3.7.13), and a nitrogenase-like enzyme, chlorophyllide a oxidoreductase (COR), respectively, to produce 3-vinyl-bacteriochlorphyllide a. The COR activity can act as a third 8VR, confirmed in vivo by the loss of 8VR activity mutants lacking bciA and genes encoding subunits of COR. In contrast to BciA and BciB, COR is a nitrogenase-like complex enzyme consisting of two separable components, the X protein (a BchX dimer) and the YZ protein (a BchY-BchZ heterotetramer). The X protein serves as the reductase component by transferring electrons from a [4Fe-4S] cluster held in the interface between the BchX homodimer and the YZ protein. The YZ protein is the catalytic component, as it accepts electrons from the X protein and transfers them to the Chlide substrate via a [4Fe-4S] cluster, to produce 3-vinyl bacteriochlorophyllide a (3 V-BChlide). 3 V-BChlide is produced via Chlide rather than via 3,8-divinyl BChlide (81,82-didehydro-3 V-BChlide) Rhodobacter capsulatus