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Literature summary for 1.3.1.33 extracted from

  • Zhang, W.; Zhong, H.; Lu, H.; Zhang, Y.; Deng, X.; Huang, K.; Duanmu, D.
    Characterization of ferredoxin-dependent biliverdin reductase PCYA1 reveals the dual function in retrograde bilin biosynthesis and interaction with light-dependent protochlorophyllide oxidoreductase LPOR in Chlamydomonas reinhardtii (2018), Front. Plant Sci., 9, 676 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
the coding regions of LPOR (amino acids 35-397) is amplified from pBDCrPCYA1-FDBR vector, cloned into pGADT7 vector, and introduced into Y187 yeast cells, recombinant expression of MBP-tagged enzyme LPOR. Yeast two-hybrid and pull down assay analyses of protein-protein interaction Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyllide a + NADP+ Chlamydomonas reinhardtii
-
 protochlorophyllide + NADPH + H+
-
 r
chlorophyllide a + NADP+ Chlamydomonas reinhardtii 4A+
-
 protochlorophyllide + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii Q39617
-
-
Chlamydomonas reinhardtii 4A+ Q39617
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyllide a + NADP+
-
 Chlamydomonas reinhardtii protochlorophyllide + NADPH + H+
-
 r
chlorophyllide a + NADP+
-
 Chlamydomonas reinhardtii 4A+ protochlorophyllide + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
Light-dependent protochlorophyllide oxidoreductase
-
 Chlamydomonas reinhardtii
LPOR
-
 Chlamydomonas reinhardtii
protochlorophyllide reductase
-
 Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Chlamydomonas reinhardtii
NADPH
-
 Chlamydomonas reinhardtii

General Information

General Information Comment Organism
metabolism ferredoxin-dependent biliverdin reductase, PCYA1 (EC 1.3.7.5), is a key enzyme involved in the biosynthesis of bilins, mechanism of bilin-mediated regulation of chlorophyll biosynthesis, and regulatory mechanisms of tetrapyrrole biosynthesis in Chlamydomonas reinhardtii, overview. Chlamydomonas PCYA1 uniquely interacts with light-dependent protochlorophyllide oxidoreductase LPOR (protochlorophyllide reductase, EC 1.3.1.33) via its FDBR domain, but not with ferredoxin:protochlorophyllide reductase DPOR (EC 1.3.7.7). This interaction is specific to Chlamydomonas since the Arabidopsis thaliana homologous proteins do not interact with each other, yeast two-hybrid and pull down assay analyses of protein-protein interaction Chlamydomonas reinhardtii
physiological function light-dependent protochlorophyllide oxidoreductase (LPOR) is a rate-limiting key chlorophyll biosynthetic enzyme Chlamydomonas reinhardtii