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Literature summary for 1.3.1.31 extracted from

  • Lee, S.; Choi, D.; Pesic, M.; Lee, Y.; Paul, C.; Hollmann, F.; Park, C.
    Cofactor-free, direct photoactivation of enoate reductases for the asymmetric reduction of C=C bonds (2017), Angew. Chem. Int. Ed. Engl., 56, 8681-8685 .
    View publication on PubMedView publication on EuropePMC
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Organism

Organism UniProt Comment Textmining
Thermus scotoductus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylcyclohexenone + NAD(P)H + H+ NAD(P)H donates two electrons and one proton directly to the enzyme-bound FMN moiety. A stepwise reduction by successive transfer of electron yields a single-electron reduced semiquinone state of FMN, which can be fully reduced by taking an additional electron. Direct transfer of photoexcited electrons from 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein (RB) to a flavin-containing OYE homologue from Thermus scotoductus upon visible light irradiation with triethanolamine (TEOA) as an electron donor is described. Successive transfer of electrons reduces the enzyme-bound FMN moiety and drives the enantioselective conversion of 2-methylcyclohexenone into enantiopure (R)-2-methycyclohexanone (ee more than 99%) Thermus scotoductus (R)-2-methylcyclohexanone + NAD(P)+
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Synonyms

Synonyms Comment Organism
OYE
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 Thermus scotoductus

Cofactor

Cofactor Comment Organism Structure
FMN NAD(P)H donates two electrons and one proton directly to the enzyme-bound FMN moiety Thermus scotoductus
NAD(P)H NAD(P)H donates two electrons and one proton directly to the enzyme-bound FMN moiety Thermus scotoductus