Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | - |
Acetoanaerobium sticklandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KBH4 | inactivates protein B, very little effect on fraction C | Acetoanaerobium sticklandii | |
LiCl | - |
Acetoanaerobium sticklandii | |
additional information | no effect: CaCl2, CoCl2, MnCl2 | Acetoanaerobium sticklandii | |
NaCl | - |
Acetoanaerobium sticklandii | |
NH2OH | inhibits protein B and fraction C | Acetoanaerobium sticklandii | |
NH4Cl | - |
Acetoanaerobium sticklandii | |
Zn(COOH)2 | - |
Acetoanaerobium sticklandii | |
ZnCl2 | - |
Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | Acetoanaerobium sticklandii | - |
glycine + phosphate + thioredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
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Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | mechanism | Acetoanaerobium sticklandii | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) | Acetoanaerobium sticklandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.5 | - |
protein B of enzyme | Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | - |
Acetoanaerobium sticklandii | glycine + phosphate + thioredoxin | - |
? | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | reduction of glycine action is coupled with formation of ATP from ADP | Acetoanaerobium sticklandii | glycine + phosphate + thioredoxin | - |
? |