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Literature summary for 1.21.4.2 extracted from

  • Bednarski, B.; Andreesen, J.R.; Pich, A.
    In vitro processing of the proproteins GrdE of protein B of glycine reductase and PrdA of D-proline reductase from Clostridium sticklandii: formation of a pyruvoyl group from a cysteine residue (2001), Eur. J. Biochem., 268, 3538-3544.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
of the pyruvoyl group-forming proproteins grdE, expression in Escherichia coli Acetoanaerobium sticklandii

Protein Variants

Protein Variants Comment Organism
C242A no cleavage on the N-terminal side of a cysteine Acetoanaerobium sticklandii
C242S cleavage on the N-terminal side of a cysteine under similar conditions with more extended half-times than wild-type enzyme Acetoanaerobium sticklandii
C242T cleavage on the N-terminal side of a cysteine under similar conditions with more extended half-times than wild-type enzyme Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21162
-
selenoprotein B, alpha,beta, 1 * 21162 + 1 * 26295, mass spectroscopy, amino acid sequence Acetoanaerobium sticklandii
26295
-
selenoprotein B, alpha,beta, 1 * 21162 + 1 * 26295, mass spectroscopy, amino acid sequence Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl phosphate + NH3 + thioredoxin disulfide + H2O Acetoanaerobium sticklandii
-
glycine + phosphate + thioredoxin
-
?
acetyl phosphate + NH3 + thioredoxin disulfide + H2O Acetoanaerobium sticklandii DSM 519
-
glycine + phosphate + thioredoxin
-
?

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii P26971 strain HF, DSM 519T from DSMZ
-
Acetoanaerobium sticklandii DSM 519 P26971 strain HF, DSM 519T from DSMZ
-

Purification (Commentary)

Purification (Comment) Organism
of pyruvoyl group-forming protein grdE Acetoanaerobium sticklandii

Reaction

Reaction Comment Organism Reaction ID
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin mechanism Acetoanaerobium sticklandii
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin cleavage mechanism for the pyruvoyl group dependent reductase starting from cysteine Acetoanaerobium sticklandii
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl phosphate + NH3 + thioredoxin disulfide + H2O
-
Acetoanaerobium sticklandii glycine + phosphate + thioredoxin
-
?
acetyl phosphate + NH3 + thioredoxin disulfide + H2O
-
Acetoanaerobium sticklandii DSM 519 glycine + phosphate + thioredoxin
-
?

Subunits

Subunits Comment Organism
dimer selenoprotein B, alpha,beta, 1 * 21162 + 1 * 26295, mass spectroscopy, amino acid sequence Acetoanaerobium sticklandii