Crystallization (Comment) | Organism |
---|---|
mutant enzymes R301K and R301A, hanging drop vapor diffusion method, using 0.1 M NaCl, 0.1 M sodium cacodylate pH 5.8, 16% PEG 6000 (w/v) (for mutant R301K) or 0.1 M ammonium acetate, 0.1 M Bis Tris pH 5.5, 17% PEG 10000 (w/v) (for mutant R301A) | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
R301A | the mutant shows about 100fold loss in kcat and a 500fold increased Km value for phosphonate. The Ki value of sulfite with the mutant is 400fold increased compared to the wild type enzyme | Pseudomonas stutzeri |
R301K | the mutant exhibits a slightly higher kcat than the wild type enzyme and a 20fold increased Km value for phosphonate | Pseudomonas stutzeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfite | competitive inhibitor | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
phosphonate | wild type enzyme, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
1.1 | - |
phosphonate | mutant enzyme R301K, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
20 | - |
phosphonate | mutant enzyme R301A, at pH 7.3 and 25°C | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonate + NAD+ + H2O | Pseudomonas stutzeri | - |
phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | Pseudomonas stutzeri WM88 | - |
phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | O69054 | - |
- |
Pseudomonas stutzeri WM88 | O69054 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography and Superdex 200 gel filtration | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonate + NAD+ + H2O | - |
Pseudomonas stutzeri | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Pseudomonas stutzeri WM88 | phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphite dehydrogenase | - |
Pseudomonas stutzeri |
PTDH | - |
Pseudomonas stutzeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas stutzeri |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00031 | - |
sulfite | wild type enzyme, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
0.078 | - |
sulfite | mutant enzyme R301K, at pH 7.3 and 25°C | Pseudomonas stutzeri |