Protein Variants | Comment | Organism |
---|---|---|
E175A | the mutation allows the enzyme to use both NAD+ and NADP+ | Pseudomonas stutzeri |
M53A | the KM values for phosphonate and NAD+ are little different from the parent enzyme, while kcat is reduced by a factor of 50 | Pseudomonas stutzeri |
M53N | the KM values for phosphonate and NAD+ are little different from the parent enzyme, while kcat is reduced by a factor of 200 | Pseudomonas stutzeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfite | - |
Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NAD+ | mutant enzyme M53A, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
0.017 | - |
phosphonate | mutant enzyme M53N, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
0.022 | - |
NAD+ | mutant enzyme E175A, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
0.028 | - |
phosphonate | mutant enzyme E175A, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
0.064 | - |
NAD+ | mutant enzyme M53N, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
0.1 | - |
phosphonate | mutant enzyme M53A, at pH 7.3 and 25°C | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonate + NAD+ + H2O | Pseudomonas stutzeri | - |
phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | Pseudomonas stutzeri WM88 | - |
phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | O69054 | - |
- |
Pseudomonas stutzeri WM88 | O69054 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonate + NAD+ + H2O | - |
Pseudomonas stutzeri | phosphate + NADH + H+ | - |
? | |
phosphonate + NAD+ + H2O | - |
Pseudomonas stutzeri WM88 | phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphite dehydrogenase | - |
Pseudomonas stutzeri |
PTDH | - |
Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
phosphonate | mutant enzyme M53N, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
0.059 | - |
phosphonate | mutant enzyme M53A, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
3.1 | - |
phosphonate | mutant enzyme E175A, at pH 7.3 and 25°C | Pseudomonas stutzeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas stutzeri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
phosphonate | mutant enzyme M53A, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
0.92 | - |
phosphonate | mutant enzyme M53N, at pH 7.3 and 25°C | Pseudomonas stutzeri | |
110 | - |
phosphonate | mutant enzyme E175A, at pH 7.3 and 25°C | Pseudomonas stutzeri |