KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
Butanoate | - |
Clostridium formicaceticum | |
1.2 | - |
4-Chlorobenzoate | - |
Clostridium formicaceticum | |
1.4 | - |
4-Fluorobenzoate | - |
Clostridium formicaceticum | |
2.1 | - |
4-Methoxybenzoate | - |
Clostridium formicaceticum | |
3.8 | - |
3-chlorobenzoate | - |
Clostridium formicaceticum | |
5 | - |
4-Methylbenzoate | - |
Clostridium formicaceticum | |
6.3 | - |
propanoate | 3-fluorobenzoate | Clostridium formicaceticum | |
6.4 | - |
propanoate | - |
Clostridium formicaceticum | |
12.8 | - |
3-methylbenzoate | - |
Clostridium formicaceticum | |
16.7 | - |
benzoate | - |
Clostridium formicaceticum | |
44.9 | - |
3-Methoxybenzoate | - |
Clostridium formicaceticum | |
50.4 | - |
3-hydroxybenzoate | - |
Clostridium formicaceticum | |
382 | - |
4-hydroxybenzoate | - |
Clostridium formicaceticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 11 mol of iron per mol of enzyme | Clostridium formicaceticum | |
Tungsten | contains 1.4 mol of tungsten per mol of enzyme | Clostridium formicaceticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
2 * 67000, SDS-PAGE | Clostridium formicaceticum |
125000 | - |
gel filtration | Clostridium formicaceticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium formicaceticum | - |
- |
- |
Oxidation Stability | Organism |
---|---|
22% loss of activity after 5 min in air, at 4°C | Clostridium formicaceticum |
Purification (Comment) | Organism |
---|---|
- |
Clostridium formicaceticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Clostridium formicaceticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-Chlorobenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 3-Chlorobenzaldehyde + carbamoyl methyl viologen | - |
? | |
3-Fluorobenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 3-Fluorobenzaldehyde + carbamoyl methyl viologen | - |
? | |
3-Hydroxybenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 3-Hydroxybenzaldehyde + carbamoyl methyl viologen | - |
? | |
3-Methoxybenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 3-Methoxybenzaldhyde + carbamoyl methyl viologen | - |
? | |
3-Methylbenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 3-Methylbenzaldehyde + carbamoyl methyl viologen | - |
? | |
4-Chlorobenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 4-Chlorobenzaldehyde + carbamoyl methyl viologen | - |
? | |
4-Fluorobenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 4-Fluorobenzaldehyde + carbamoyl methyl viologen | - |
? | |
4-Hydroxybenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 4-Hydroxybenzaldehyde + carbamoyl methyl viologen | - |
? | |
4-Methoxybenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 4-Methoxybenzaldehyde + carbamoyl methyl viologen | - |
? | |
4-Methylbenzoate + reduced carbamoyl methyl viologen | - |
Clostridium formicaceticum | 4-Methylbenzaldehyde + carbamoyl methyl viologen | - |
? | |
Benzaldehyde + carbamoyl methyl viologen | only reverse reaction described | Clostridium formicaceticum | Benzoate + reduced carbamoyl methyl viologen | - |
? | |
Butanal + NH2CO-methyl viologen | only reverse reaction described | Clostridium formicaceticum | Butanoate + reduced NH2CO-methyl viologen | - |
? | |
Propanal + benzyl viologen | only reverse reaction described | Clostridium formicaceticum | Propanoate + reduced benzyl viologen | - |
? | |
Propanal + methyl viologen | only reverse reaction described | Clostridium formicaceticum | Propanoate + reduced methyl viologen | - |
? | |
Propanal + NH2CO-methyl viologen | only reverse reaction described | Clostridium formicaceticum | Propanoate + reduced carbamoyl methyl viologen | - |
? | |
Propanal + reduced 1,1',2,2'-tetramethylviologen | - |
Clostridium formicaceticum | Propanoate + 1,1',2,2'-tetramethylviologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 67000, SDS-PAGE | Clostridium formicaceticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
aldehyde dehydrogenation | Clostridium formicaceticum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 60% of maximal activity at 30°C and 50°C, inactive at 60°C, aldehyde dehydrogenation | Clostridium formicaceticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of propanoate or benzoate | Clostridium formicaceticum |
9 | - |
butanal and NH2-CO-methyl viologen as substrates | Clostridium formicaceticum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | pH 6.0: optimum, pH 7.0: 25% of maximal activity, reduction of propanoate or benzoate | Clostridium formicaceticum |
8 | 10 | about 90% of maximal activity, butanal and NH2-CO-methyl viologen as substrates | Clostridium formicaceticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pterin | contains 1.6 mol of pterin-6-carboxylic acid per mol of enzyme | Clostridium formicaceticum |