Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tungsten | stimulates cell growth when added to the medium | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
D-Glyceraldehyde-3-phosphate | with ferredoxin as electron acceptor, pH 8.4, 70°C | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1 [4Fe-4S]2+ cluster per enzyme molecule | Pyrococcus furiosus | |
Fe2+ | 5-6 iron g-atoms per mol of enzyme | Pyrococcus furiosus | |
Mg2+ | contains 1 molecule per enzyme | Pyrococcus furiosus | |
Tungsten | 1 tungsten center per molecule of enzyme | Pyrococcus furiosus | |
Zn2+ | contains 2 molecules per enzyme | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | Pyrococcus furiosus | - |
3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Oxidation Stability | Organism |
---|---|
O2-sensitive, t1/2 at 23°C: 6 h | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
to homogeneity | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
strictly anaerobic assay conditions | Pyrococcus furiosus |
25 | - |
purified enzyme | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen | artificial electron acceptor | Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced benzyl viologen | - |
? | |
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? | |
additional information | no activity with NAD(P)+ as electron acceptor | Pyrococcus furiosus | ? | - |
? | |
additional information | no activity with formaldehyde, propionaldehyde, phenylacetaldehyde, butyraldehyde, crotonaldehyde, acetaldehyde, dihydroxyacetone phosphate, glyceraldehyde, benzaldehyde, glucose, glucose 6-phosphate, or glyoxylate | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 73000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
glyceraldehyde-3-phosphate ferredoxin oxidoreductase | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
t1/2: 15 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Pyrococcus furiosus | |
tungsten-molybdopterin | tungstopterin center | Pyrococcus furiosus | |
tungsten-molybdopterin | structure | Pyrococcus furiosus |