KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00295 | - |
methylene blue | Ag+-substituted enzyme, at pH 7.0 and 25°C | Afipia carboxidovorans | |
0.0107 | - |
methylene blue | native enzyme, at pH 7.0 and 25°C | Afipia carboxidovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the native enzyme contains copper in the active site | Afipia carboxidovorans | |
[2Fe-2S]-center | the small subunit contains two [2Fe-2S] centers | Afipia carboxidovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + acceptor | Afipia carboxidovorans | - |
CO2 + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Afipia carboxidovorans | P19919 and P19920 and P19921 | P19919 large subunit, P19920 medium subunit, P19921 small subunit | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + acceptor | - |
Afipia carboxidovorans | CO2 + reduced acceptor | - |
? | |
CO + H2O + methylene blue | - |
Afipia carboxidovorans | CO2 + reduced methylene blue | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | - |
Afipia carboxidovorans |
Synonyms | Comment | Organism |
---|---|---|
CODH | - |
Afipia carboxidovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.2 | - |
methylene blue | Ag+-substituted enzyme, at pH 7.2 and 25°C | Afipia carboxidovorans | |
93.3 | - |
methylene blue | native enzyme, at pH 7.2 and 25°C | Afipia carboxidovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the medium subunit contains FAD | Afipia carboxidovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2780 | - |
methylene blue | Ag+-substituted enzyme, at pH 7.2 and 25°C | Afipia carboxidovorans | |
8700 | - |
methylene blue | native enzyme, at pH 7.2 and 25°C | Afipia carboxidovorans |