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Literature summary for 1.2.7.4 extracted from

  • Wilcoxen, J.; Snider, S.; Hille, R.
    Substitution of silver for copper in the binuclear Mo/Cu center of carbon monoxide dehydrogenase from Oligotropha carboxidovorans (2011), J. Am. Chem. Soc., 133, 12934-12936.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00295
-
methylene blue Ag+-substituted enzyme, at pH 7.0 and 25°C Afipia carboxidovorans
0.0107
-
methylene blue native enzyme, at pH 7.0 and 25°C Afipia carboxidovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the native enzyme contains copper in the active site Afipia carboxidovorans
[2Fe-2S]-center the small subunit contains two [2Fe-2S] centers Afipia carboxidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO + H2O + acceptor Afipia carboxidovorans
-
CO2 + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Afipia carboxidovorans P19919 and P19920 and P19921 P19919 large subunit, P19920 medium subunit, P19921 small subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + acceptor
-
Afipia carboxidovorans CO2 + reduced acceptor
-
?
CO + H2O + methylene blue
-
Afipia carboxidovorans CO2 + reduced methylene blue
-
?

Subunits

Subunits Comment Organism
heterohexamer
-
Afipia carboxidovorans

Synonyms

Synonyms Comment Organism
CODH
-
Afipia carboxidovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.2
-
methylene blue Ag+-substituted enzyme, at pH 7.2 and 25°C Afipia carboxidovorans
93.3
-
methylene blue native enzyme, at pH 7.2 and 25°C Afipia carboxidovorans

Cofactor

Cofactor Comment Organism Structure
FAD the medium subunit contains FAD Afipia carboxidovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2780
-
methylene blue Ag+-substituted enzyme, at pH 7.2 and 25°C Afipia carboxidovorans
8700
-
methylene blue native enzyme, at pH 7.2 and 25°C Afipia carboxidovorans