Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli BL21(DE3) cells | Gelatoporia subvermispora |
Protein Variants | Comment | Organism |
---|---|---|
A242E | the mutant shows 5% of the wild type oxidase activity | Gelatoporia subvermispora |
D241A | the mutant contains 17% of the Mn2+ content of the recombinant wild type enzyme and 7% of the wild type oxidase activity | Gelatoporia subvermispora |
D241S | inactive | Gelatoporia subvermispora |
R169K | inactive | Gelatoporia subvermispora |
R349K | inactive | Gelatoporia subvermispora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
oxalate | wild type enzyme, pH and temperature not specified in the publication | Gelatoporia subvermispora | |
1.1 | - |
oxalate | mutant enzyme D241A, pH and temperature not specified in the publication | Gelatoporia subvermispora | |
2 | - |
oxalate | mutant enzyme A242E, pH and temperature not specified in the publication | Gelatoporia subvermispora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | dependent on | Gelatoporia subvermispora | |
additional information | not activated by Mg2+, Co2+, Zn2+, Ni2+, and Fe2+ | Gelatoporia subvermispora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + O2 + 2 H+ | Gelatoporia subvermispora | - |
CO2 + 2 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gelatoporia subvermispora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anion exchange column chromatography and butyl Sepharose column chromatography | Gelatoporia subvermispora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + O2 + 2 H+ | - |
Gelatoporia subvermispora | CO2 + 2 H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OxOx | - |
Gelatoporia subvermispora |