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Literature summary for 1.2.3.3 extracted from

  • Tittmann, K.; Proske, D.; Spinka, M.; Ghisla, S.; Rudolph, R.; Hubner, G.; Kern, G.
    Activation of thiamin diphosphate and FAD in the phosphate-dependent pyruvate oxidase from Lactobacillus plantarum (1998), J. Biol. Chem., 273, 12929-12934.
    View publication on PubMed
Search for more literature for 1.2.3.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2,6-dichlorophenolindophenol artificial electron acceptor Lactiplantibacillus plantarum
0.07
-
 oxygen
-
 Lactiplantibacillus plantarum
0.4
-
 pyruvate electron acceptor: oxygen Lactiplantibacillus plantarum
1.6
-
 pyruvate electron acceptor: 2,6-dichlorophenolindophenol Lactiplantibacillus plantarum
2.3
-
 phosphate electron acceptor: oxygen Lactiplantibacillus plantarum
4.5
-
 phosphate electron acceptor: 2,6-dichlorophenolindophenol Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for binding of FAD and thiamine diphosphate Lactiplantibacillus plantarum
Mn2+ required for binding of FAD and thiamine diphosphate Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + phosphate + O2 + H2O Lactiplantibacillus plantarum 5 step catalytic reaction acetyl phosphate + CO2 + H2O2
-
 ir

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + phosphate + 2,6-dichlorophenolindophenol + H2O
-
 Lactiplantibacillus plantarum acetyl phosphate + CO2 + reduced 2,6-dichlorophenolindophenol + H2O2
-
 ?
pyruvate + phosphate + O2 + H2O 5step catalytic reaction Lactiplantibacillus plantarum acetyl phosphate + CO2 + H2O2
-
 ir
pyruvate + phosphate + O2 + H2O 5 step catalytic reaction Lactiplantibacillus plantarum acetyl phosphate + CO2 + H2O2
-
 ir

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.6
-
 pyruvate electron acceptor: O2 Lactiplantibacillus plantarum
17.9
-
 pyruvate electron acceptor: 2,6-dichlorophenolindophenol Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
FAD binding of FAD in a monophasic, pseudo first order process Lactiplantibacillus plantarum
thiamine diphosphate indirect activation by FAD that is mediated by the protein moiety Lactiplantibacillus plantarum