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Literature summary for 1.2.1.99 extracted from

  • Raj, S.M.; Rathnasingh, C.; Jo, J.-E.; Park, S.
    Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain (2008), Proc. Biochem., 32, 1440-1446.
No PubMed abstract available

Application

Application Comment Organism
synthesis 3-hydroxypropionic acid is an important compound from which several commodity and specialty chemicals can be generated. The recombinant strain SH254 producing 3-hydroxypropionate from glycerol is developed by cloning of dhaB and aldH genes in Escherichia coli BL21 under different regulatory promoters. The recombinant SH254 can accumulate 3-hydroxypropionic acid from glycerol at 6.5 mmol/l in 30 h Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
the recombinant strain SH254 producing 3-hydroxypropionate from glycerol is developed by cloning of dhaB and aldH genes in Escherichia coli BL21 under different regulatory promoters Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropionaldehyde + NAD+ + H2O Escherichia coli
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3-hydroxypropanoate + NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P23883
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-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropionaldehyde + NAD+ + H2O
-
Escherichia coli 3-hydroxypropanoate + NADH + 2 H+
-
?