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Literature summary for 1.2.1.99 extracted from

  • Raj, S.; Rathnasingh, C.; Jung, W.; Selvakumar, E.; Park, S.
    A novel NAD+-dependent aldehyde dehydrogenase encoded by the puuC gene of Klebsiella pneumoniae DSM 2026 that utilizes 3-hydroxypropionaldehyde as a substrate (2010), Biotechnol. Bioprocess Eng., 15, 131-138.
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Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, activity increases by 61% Klebsiella pneumoniae
dithiothreitol 1 mM, activity increases by 47.5% Klebsiella pneumoniae
Semicarbazide hydrochloride 1 mM, activity increases by 19% Klebsiella pneumoniae

Application

Application Comment Organism
synthesis PuuC is an active enzyme that can be utilized effectively for several purposes, including biological production of 3-hydroxypropionate Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition Klebsiella pneumoniae
Co2+ 1 mM, 11% inhibition Klebsiella pneumoniae
Cu2+ 1 mM, complete inhibition Klebsiella pneumoniae
EDTA 1 mM, 8.3% inhibition Klebsiella pneumoniae
Fe2+ 1 mM, 73.6% inhibition Klebsiella pneumoniae
Hg2+ 1 mM, complete inhibition Klebsiella pneumoniae
Li+ 1 mM, 9.4% inhibition Klebsiella pneumoniae
Mg2+ 1 mM, 6.3% inhibition Klebsiella pneumoniae
NaCl mM, 11% inhibition Klebsiella pneumoniae
NH4Cl 8% inhibition Klebsiella pneumoniae
Ni2+ 1 mM, 10.5% inhibition Klebsiella pneumoniae
Zn2+ 1 mM, 64% inhibition Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 Valeraldehyde pH 8.0, 45°C Klebsiella pneumoniae
0.21
-
 Isovaleraldehyde pH 8.0, 45°C Klebsiella pneumoniae
0.26
-
 Butyraldehyde pH 8.0, 45°C Klebsiella pneumoniae
0.48
-
 3-hydroxypropionaldehyde pH 8.0, 45°C Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53800
-
 x * 53800, SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropionaldehyde + NAD+ + H2O Klebsiella pneumoniae the enzyme might play a role in putresine and propionate metabolism 3-hydroxypropanoate + NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae DSM 2026
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.25
-
 Vmax: 3-hydroxypropionaldehyde, pH 8.0, 45°C Klebsiella pneumoniae
28.61
-
 Vmax: isovaleraldehyde, pH 8.0, 45°C Klebsiella pneumoniae
33.26
-
 Vmax: valeraldehyde, pH 8.0, 45°C Klebsiella pneumoniae
34.08
-
 Vmax: butyraldehyde, pH 8.0, 45°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-furaldehyde + NAD+ + H2O 22% of the activity with 3-hydroxypropionaldehyde Klebsiella pneumoniae furan-2-carboxylate + NADH + 2 H+
-
 ?
3-hydroxypropionaldehyde + NAD+ + H2O
-
 Klebsiella pneumoniae 3-hydroxypropanoate + NADH + 2 H+
-
 ?
3-hydroxypropionaldehyde + NAD+ + H2O the enzyme might play a role in putresine and propionate metabolism Klebsiella pneumoniae 3-hydroxypropanoate + NADH + 2 H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Klebsiella pneumoniae acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O 35% of the activity with 3-hydroxypropionaldehyde Klebsiella pneumoniae benzoate + NADH + H+
-
 ?
butyraldehyde + NAD+ + H2O
-
 Klebsiella pneumoniae butyrate + NADH + 2 H+
-
 ?
isovaleraldehyde + NAD+ + H2O
-
 Klebsiella pneumoniae 3-methylbutanoate + NADH + 2 H+
-
 ?
additional information no activity with formaldehyde Klebsiella pneumoniae ?
-
 ?
propionaldehyde + NAD+ + H2O
-
 Klebsiella pneumoniae propionate + NADH + 2 H+
-
 ?
valeraldehyde + NAD+ + H2O most preferred substrate Klebsiella pneumoniae pentanoate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 53800, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
PuuC
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 30% of maximal activity, 60°C: about 35% of maximal activity Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 becomes unstable at 60°C Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.81
-
 3-hydroxypropionaldehyde pH 8.0, 45°C Klebsiella pneumoniae
25.47
-
 Isovaleraldehyde pH 8.0, 45°C Klebsiella pneumoniae
29.61
-
 Valeraldehyde pH 8.0, 45°C Klebsiella pneumoniae
30.34
-
 Butyraldehyde pH 8.0, 45°C Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred coenzyme over NADP+ Klebsiella pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
41.44
-
 3-hydroxypropionaldehyde pH 8.0, 45°C Klebsiella pneumoniae
117.2
-
 Butyraldehyde pH 8.0, 45°C Klebsiella pneumoniae
121.9
-
 Isovaleraldehyde pH 8.0, 45°C Klebsiella pneumoniae
207.1
-
 Valeraldehyde pH 8.0, 45°C Klebsiella pneumoniae