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Literature summary for 1.2.1.92 extracted from

  • Tsevelkhorloo, M.; Kim, S.H.; Kang, D.K.; Lee, C.R.; Hong, S.K.
    NADP+-dependent dehydrogenase SCO3486 and cycloisomerase SCO3480 key enzymes for 3,6-anhydro-L-galactose catabolism in Streptomyces coelicolor A3(2) (2021), J. Microbiol. Biotechnol., 31, 756-763 .
    View publication on PubMed
Search for more literature for 1.2.1.92

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 80% residual activity at 5 mM Streptomyces coelicolor
Co2+ about 20% residual activity at 5 mM Streptomyces coelicolor
Cu2+ about 45% residual activity at 5 mM Streptomyces coelicolor
EDTA
-
 Streptomyces coelicolor
K+ about 70% residual activity at 5 mM Streptomyces coelicolor
Mg2+ about 85% residual activity at 5 mM Streptomyces coelicolor
Mn2+ about 50% residual activity at 5 mM Streptomyces coelicolor
Na+ about 90% residual activity at 5 mM Streptomyces coelicolor
Ni2+ about 20% residual activity at 5 mM Streptomyces coelicolor
Zn2+ about 50% residual activity at 5 mM Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 167% activity at 5 mM Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O Streptomyces coelicolor
-
 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O Streptomyces coelicolor A3(2)
-
 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9RKF1
-
-
Streptomyces coelicolor A3(2) Q9RKF1
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon metal affinity column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O
-
 Streptomyces coelicolor 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O 100% activity Streptomyces coelicolor 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O
-
 Streptomyces coelicolor A3(2) 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O 100% activity Streptomyces coelicolor A3(2) 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
D-fructose + NADP+ + H2O about 55% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
D-fructose + NADP+ + H2O about 55% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor A3(2) ?
-
 ?
D-galactose + NADP+ + H2O about 50% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
D-galactose + NADP+ + H2O about 50% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor A3(2) ?
-
 ?
D-glucose + NADP+ + H2O 20% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
D-glucose + NADP+ + H2O 20% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor A3(2) ?
-
 ?
D-glyceraldehyde + NADP+ + H2O less than 20% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
D-lyxose + NADP+ + H2O about 15% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
D-ribose + NADP+ + H2O about 45% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
L-fucose + NADP+ + H2O 25% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
L-glyceraldehyde + NADP+ + H2O less than 10% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?
L-rhamnose + NADP+ + H2O less than 1% activity compared to 3,6-anhydro-alpha-L-galactopyranose Streptomyces coelicolor ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Streptomyces coelicolor
? x * 55718, calculated from amino acid sequence Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
AHG dehydrogenase
-
 Streptomyces coelicolor
SCO3486
-
 Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces coelicolor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 the enzyme maintains above 50% activity between 40 and 70°C Streptomyces coelicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 the enzyme is stable up to 40°C but gradually loses its activity at temperatures above 50°C Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces coelicolor

pH Range

pH Minimum pH Maximum Comment Organism
5 8 the enzyme shows 44% and 42% activity at pH 5.0 and 8.0, respectively Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Streptomyces coelicolor