Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 132.2% activity at 1 mM | Vibrio sp. EJY3 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 80.8% residual activity at 1 mM | Vibrio sp. EJY3 | |
Cd2+ | complete inhibition at 1 mM | Vibrio sp. EJY3 | |
Co2+ | complete inhibition at 1 mM | Vibrio sp. EJY3 | |
Cu2+ | complete inhibition at 1 mM | Vibrio sp. EJY3 | |
Mn2+ | 68.2% residual activity at 1 mM | Vibrio sp. EJY3 | |
additional information | not inhibited by EDTA | Vibrio sp. EJY3 | |
Ni2+ | complete inhibition at 1 mM | Vibrio sp. EJY3 | |
Zn2+ | complete inhibition at 1 mM | Vibrio sp. EJY3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
3,6-anhydro-alpha-L-galactopyranose | at pH 8.0 and 20°C | Vibrio sp. EJY3 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+, Na+ and K+ have no effect on the enzyme activity | Vibrio sp. EJY3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O | Vibrio sp. EJY3 | the enzyme is highly specific to 3,6-anhydro-alpha-L-galactopyranose | 3,6-anhydro-L-galactonate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. EJY3 | H2IFE7 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Vibrio sp. EJY3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O | the enzyme is highly specific to 3,6-anhydro-alpha-L-galactopyranose | Vibrio sp. EJY3 | 3,6-anhydro-L-galactonate + NADPH + H+ | - |
? | |
additional information | no activity with D-glucose, D-galactose, D-fructose, sucrose, maltose, arabinose, xylose, agarohexose, agarotetrose and neoagarobiose | Vibrio sp. EJY3 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
AHGD | - |
Vibrio sp. EJY3 |
anhydrogalactose dehydrogenase | - |
Vibrio sp. EJY3 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Vibrio sp. EJY3 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | the enzyme shows 50% activity after 12 h at 20°C and pH 8.0. At 50°C, the half-life of the enzyme is less than 10 min | Vibrio sp. EJY3 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
3,6-anhydro-alpha-L-galactopyranose | at pH 8.0 and 20°C | Vibrio sp. EJY3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Vibrio sp. EJY3 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 12 | the enzyme shows over 90% of its maximum within a small pH range from 8.0 to 11.0 and more than 60% activity between pH 6.0 and 12.0. At pH values lower than 5.0, activity of the enzyme is drastically reduced | Vibrio sp. EJY3 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
the enzyme shows 50% activity after 12 h at 20°C and pH 8.0 | Vibrio sp. EJY3 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Vibrio sp. EJY3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
3,6-anhydro-alpha-L-galactopyranose | at pH 8.0 and 20°C | Vibrio sp. EJY3 |