Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose 1-phosphate | - |
Thermococcus kodakarensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) CodonPlus RIL cells | Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glyceraldehyde 3-phosphate | substrate inhibition | Thermococcus kodakarensis | |
L-Glyceraldehyde 3-phosphate | non-competitive inhibition | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55486 | - |
4 * 55486, calculated from amino acid sequence | Thermococcus kodakarensis |
230000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glyceraldehyde 3-phosphate + NADP+ + H2O | Thermococcus kodakarensis | - |
3-phospho-DL-glycerate + NADPH + H+ | - |
? | |
additional information | Thermococcus kodakarensis | the enzyme does not react with formaldehyde, acetaldehyde, propionaldehyde, butylaldehyde, valeraldehyde, caproaldehyde, betaine aldehyde, glycolaldehyde, succinic semialdehyde or glyceraldehyde | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat treatment (85°C, 30 min), anion exchange chromatography and gel filtration | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glyceraldehyde 3-phosphate + NADP+ + H2O | - |
Thermococcus kodakarensis | 3-phospho-DL-glycerate + NADPH + H+ | - |
? | |
additional information | the enzyme does not react with formaldehyde, acetaldehyde, propionaldehyde, butylaldehyde, valeraldehyde, caproaldehyde, betaine aldehyde, glycolaldehyde, succinic semialdehyde or glyceraldehyde | Thermococcus kodakarensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 55486, calculated from amino acid sequence | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
GAPN | - |
Thermococcus kodakarensis |
non-phosphorylating GAP dehydrogenase | - |
Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme activity is observed with both NAD+ and NADP+, but NADP+ is the preferred cofactor | Thermococcus kodakarensis | |
NADP+ | enzyme activity is observed with both NAD+ and NADP+, but NADP+ is the preferred cofactor | Thermococcus kodakarensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
36.5 | - |
D-glyceraldehyde 3-phosphate | in 100 mM MES/KOH (pH 7.0), at 70°C | Thermococcus kodakarensis |
General Information | Comment | Organism |
---|---|---|
malfunction | DELTAgapN cells do not grow under glycolytic conditions | Thermococcus kodakarensis |
metabolism | the enzyme plays a key role in providing NADPH under glycolytic conditions | Thermococcus kodakarensis |