Literature summary for 1.2.1.89 extracted from

Iermak, I.; Degtjarik, O.; Steffler, F.; Sieber, V.; Kuta Smatanova, I.
Crystallization behaviour of glyceraldehyde dehydrogenase from Thermoplasma acidophilum (2015), Acta Crystallogr. F Struct. Biol. Commun., 71, 1475-1480 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thermoplasma acidophilum

Crystallization (Commentary)

Crystallization (Comment)	Organism
wild type enzyme, sitting drop vapor diffusion method, using 50 mM MOPS, 50 mM sodium HEPES pH 7.5, 20% (w/v) ethylene glycol, 10% (w/v) PEG 8000, 20 mM glutamic acid (racemic), 20 mM glycine, 20 mM serine (racemic), 20 mM alanine (racemic), 20 mM lysine-HCl (racemic). Mutant enzyme F34M/S405N, sitting drop vapor diffusion method, using 50 mM MES, 50 mM imidazole pH 6.5, 8.3% (w/v) PEG 8000, 16.7% (w/v) ethylene glycol, 20 mM sodium formate, 20 mM sodium citrate, 20 mM sodium oxamate, 20 mM ammonium acetate, 20 mM sodium potassium tartrate	Thermoplasma acidophilum

Crystallization (Comment)

Organism

wild type enzyme, sitting drop vapor diffusion method, using 50 mM MOPS, 50 mM sodium HEPES pH 7.5, 20% (w/v) ethylene glycol, 10% (w/v) PEG 8000, 20 mM glutamic acid (racemic), 20 mM glycine, 20 mM serine (racemic), 20 mM alanine (racemic), 20 mM lysine-HCl (racemic). Mutant enzyme F34M/S405N, sitting drop vapor diffusion method, using 50 mM MES, 50 mM imidazole pH 6.5, 8.3% (w/v) PEG 8000, 16.7% (w/v) ethylene glycol, 20 mM sodium formate, 20 mM sodium citrate, 20 mM sodium oxamate, 20 mM ammonium acetate, 20 mM sodium potassium tartrate

Thermoplasma acidophilum

Protein Variants

Protein Variants	Comment	Organism
F34M/S405N	the mutant shows a slight increase in NAD+ acceptance and a 9fold improvement in catalytic efficiency with NAD+ compared with the wild type enzyme	Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde + NADP+ + H2O	Thermoplasma acidophilum	-	D-glycerate + NADPH + H+	-	?
D-glyceraldehyde + NADP+ + H2O	Thermoplasma acidophilum ATCC 25905	-	D-glycerate + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	Q9HK01	-	-
Thermoplasma acidophilum ATCC 25905	Q9HK01	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography	Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-glyceraldehyde + NADP+ + H2O	-	Thermoplasma acidophilum	D-glycerate + NADPH + H+	-	?
D-glyceraldehyde + NADP+ + H2O	-	Thermoplasma acidophilum ATCC 25905	D-glycerate + NADPH + H+	-	?
additional information	NAD+ is a poor substrate	Thermoplasma acidophilum	?	-	-
additional information	NAD+ is a poor substrate	Thermoplasma acidophilum ATCC 25905	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 56446, calculated from amino acid sequence	Thermoplasma acidophilum

Synonyms

Synonyms	Comment	Organism
ALDH	-	Thermoplasma acidophilum
glyceraldehyde dehydrogenase	-	Thermoplasma acidophilum

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Thermoplasma acidophilum