Any feedback?
Literature summary for 1.2.1.88 extracted from
- DELTA1-Pyrroline-5-carboxylate dehydrogenase from cultured cells of potato. Purification and properties (1997), Plant Physiol., 113, 1413-1418.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chloride | - |
Solanum tuberosum |  |
| sulfate | no inhibition | Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
NAD+ | - |
Solanum tuberosum | |
| 0.46 | - |
1-pyrroline-5-carboxylate | - |
Solanum tuberosum | |
| 0.662 | - |
NADP+ | - |
Solanum tuberosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
4 * 60000, SDS-PAGE | Solanum tuberosum |
| 240000 | - |
gel filtration | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NAD+ + H2O | Solanum tuberosum | - |
L-glutamate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 44.15 | - |
- |
Solanum tuberosum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 0.2 M potsassium phosphate or 0.2 M KCl, pH 7.4, 45 days, 70-80% activity remains | Solanum tuberosum |
| 0°C, 25 mM Hepes-KOH, pH 7.4, 0.5 mM dithiothreitol, 1 mM MgCl2, 1% polyvinyl polypyrrolidone, loss of 90% activity after 15 days | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Solanum tuberosum | L-glutamate + NADH | - |
? | |
| 1-pyrroline-5-carboxylate + NADP+ + H2O | - |
Solanum tuberosum | L-glutamate + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, SDS-PAGE | Solanum tuberosum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P5C dehydrogenase | - |
Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
- |
Solanum tuberosum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 37.5 | - |
Solanum tuberosum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
half-life 44 min | Solanum tuberosum |
| 50 | - |
half-life 30 min | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8.3 | - |
Solanum tuberosum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | preferentially | Solanum tuberosum | |
| NADP+ | 60% of the activity with NAD+ | Solanum tuberosum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
