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Literature summary for 1.2.1.88 extracted from

  • Smith, D.D.S.; Wood, N.J.; Hodgson, D.A.
    Interaction between primary and secondary metabolism in Streptomyces coelicolor A3(2): role of pyrroline-5-carboxylate dehydrogenase (1995), Microbiology, 141, 1739-1744.
No PubMed abstract available
Search for more literature for 1.2.1.88

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect of proline on activity Streptomyces coelicolor

Application

Application Comment Organism
medicine investigation of regulation of antibiotic production, i.e. undecylprodigiosin and related compounds, in secondary metabolism from proline as amino acid precursor Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
L-Glu very weak inhibitor Streptomyces coelicolor
additional information no effect of proline on activity Streptomyces coelicolor
NADH product inhibition Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 NAD+
-
 Streptomyces coelicolor
0.109
-
 1-pyrroline-5-carboxylate
-
 Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 4 * 68000, SDS-PAGE Streptomyces coelicolor
265000 305000 native PAGE and gel filtration Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-pyrroline-5-carboxylate + NAD+ + H2O Streptomyces coelicolor
-
 L-glutamate + NADH
-
 ?
1-pyrroline-5-carboxylate + NAD+ + H2O Streptomyces coelicolor A3(2)
-
 L-glutamate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces coelicolor

Reaction

Reaction Comment Organism Reaction ID
L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH + H+ induction by proline, activation on gene transcription level Streptomyces coelicolor
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Streptomyces coelicolor L-glutamate + NADH
-
 ?
1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Streptomyces coelicolor A3(2) L-glutamate + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 68000, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
P5C dehydrogenase
-
 Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
 1-pyrroline-5-carboxylate
-
 Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Streptomyces coelicolor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
 NADH product inhibition, with NAD+ Streptomyces coelicolor
0.77
-
 NADH product inhibition, with 1-pyrroline-5-carboxylate Streptomyces coelicolor