Cloned (Comment) | Organism |
---|---|
carD expressed in Escherichia coli BL21 strain | Fusarium fujikuroi |
generation of an Escherichia coli strain producing beta-apo-4'-carotenal, which is converted into neurosporaxanthin upon expression of carD | Fusarium fujikuroi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-apo-4'-carotenal + NAD+ + H2O | Fusarium fujikuroi | carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal | beta-apo-4'-carotenoic acid + NADH + H+ | beta-apo-4'-carotenoic acid i.e. neurosporaxanthin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium fujikuroi | F6IBC7 | - |
- |
Purification (Comment) | Organism |
---|---|
partial purification by generating of a crude lysate for in vitro assays | Fusarium fujikuroi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
apo-8'-lycopenal + NAD+ + H2O | - |
Fusarium fujikuroi | apo-8'-lycopenoic acid + NADH + 2 H+ | - |
? | |
apo-8'-lycopenal + NAD+ + H2O | - |
Fusarium fujikuroi | apo-8'-lycopenoic acid + NADH + H+ | - |
? | |
beta-apo-10'-carotenal + NAD+ + H2O | - |
Fusarium fujikuroi | beta-apo-10'-carotenoic acid + NADH + H+ | - |
? | |
beta-apo-10'-carotenal + NAD+ + H2O | - |
Fusarium fujikuroi | beta-apo-10'-carotenoic acid + NADH + 2 H+ | - |
? | |
beta-apo-4'-carotenal + NAD+ + H2O | carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal | Fusarium fujikuroi | beta-apo-4'-carotenoic acid + NADH + H+ | beta-apo-4'-carotenoic acid i.e. neurosporaxanthin | ? | |
beta-apo-4'-carotenal + NAD+ + H2O | - |
Fusarium fujikuroi | neurosporaxanthin + NADH + 2 H+ | - |
? | |
beta-apo-8'-carotenal + NAD+ + H2O | - |
Fusarium fujikuroi | beta-apo-8'-carotenoic acid + NADH + H+ | - |
? | |
beta-apo-8'-carotenal + NAD+ + H2O | - |
Fusarium fujikuroi | beta-apo-8'-carotenoic acid + NADH + 2 H+ | - |
? | |
additional information | no substrate: retinal | Fusarium fujikuroi | ? | - |
? | |
additional information | C20-retinal i.e. beta-apo-15'-carotenal is not converted by carD enzymatic activity, indicating the requirement for a minimal length of the substrate chain | Fusarium fujikuroi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CarD | - |
Fusarium fujikuroi |
Organism | Comment | Expression |
---|---|---|
Fusarium fujikuroi | CarD mRNA content is enhanced by light, and CarD mRNA levels are higher in carotenoid-overproducing mutants | up |
Fusarium fujikuroi | carD mRNA levels increase about 3fold after 30 min of illumination | up |
General Information | Comment | Organism |
---|---|---|
physiological function | targeted disruption of CarD in a Gibberella fujikuroi carotenoid-overproducing strain results in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal, its derivative beta-apo-4'-carotenol, and minor amounts of other carotenoids. Strains harboring the CarD mutation acquire a yellowish pigmentation | Fusarium fujikuroi |