Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | maximum activity at 0.5 M | Staphylococcus aureus | |
NaCl | maximum activity at 0.5 M | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)/pMAGIC cells | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 200 mM MgCl2,100 mM Tris pH 8.5, 20% (w/v) PEG 8000 | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
G234S | the mutant shows increased activity and reduced substrate inhibition compared to the wild type enzyme | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Betaine aldehyde | strongly inhibited by betaine aldehyde at concentrations of more than 0.15 mM | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Betaine aldehyde | at pH 8.0 and 30°C | Staphylococcus aureus | |
0.43 | - |
NAD+ | at pH 8.0 and 30°C | Staphylococcus aureus | |
2.26 | - |
NADP+ | at pH 8.0 and 30°C | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | Staphylococcus aureus |
215000 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | Staphylococcus aureus | - |
betaine + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q9L4P8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | - |
Staphylococcus aureus | betaine + NADH + H+ | - |
? | |
betaine aldehyde + NADP+ + H2O | the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher | Staphylococcus aureus | betaine + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 60000, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
BADH | - |
Staphylococcus aureus |
SACOL2628 | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
NADP+ | at pH 8.0 and 30°C | Staphylococcus aureus | |
6.18 | - |
NAD+ | at pH 8.0 and 30°C | Staphylococcus aureus | |
11 | - |
Betaine aldehyde | at pH 8.0 and 30°C | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher | Staphylococcus aureus | |
NADP+ | the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher | Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
Betaine aldehyde | at pH 8.0 and 30°C | Staphylococcus aureus |