Application | Comment | Organism |
---|---|---|
medicine | the enzyme could be a target for antibiotic design | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas aeruginosa |
plasmid pCALbetB containing the full sequence of the gene betB that encodes PaBADH is used for the expression of the enzyme in Escherichia coli cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
BADH in the presence of 2-mercaptoethanol, glycerol, NADP+ and 150 mM K+, hanging drop vapor diffusion method, using 85 mM HEPESNaOH, pH 7.5, 8.5% (v/v) isopropanol, 17% (w/v) polyethylene glycol 4000 and 15% (v/v) glycerol, at 18°C | Pseudomonas aeruginosa |
the three dimensional structure of betaine aldehyde dehydrogenase, in complex with glycerol, NADP+ and K+ ions, is determined at 2.1 A resolution | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Pseudomonas aeruginosa | |
K+ | the enzyme requires K+ ions for stability and possesses two K+ binding sites per subunit | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD(P)+ + H2O | Pseudomonas aeruginosa | - |
glycine betaine + NAD(P)H + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa | Q9HTJ1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
by a two step procedure | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD(P)+ + H2O | - |
Pseudomonas aeruginosa | glycine betaine + NAD(P)H + H+ | - |
ir | |
betaine aldehyde + NAD+ + H2O | - |
Pseudomonas aeruginosa | betaine + NADH + H+ | - |
ir | |
betaine aldehyde + NADP+ + H2O | - |
Pseudomonas aeruginosa | betaine + NADPH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase | - |
Pseudomonas aeruginosa |
BADH | - |
Pseudomonas aeruginosa |
betaine aldehyde dehydrogenase | - |
Pseudomonas aeruginosa |
betaine aldehyde: NAD(P)+ oxidoreductase | - |
Pseudomonas aeruginosa |
PaBADH | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | PaBADH uses NADP+ with the same efficiency as NAD+ | Pseudomonas aeruginosa | |
NAD+ | BADH uses NAD+ with the same efficiency as NADP+ | Pseudomonas aeruginosa | |
NADP+ | PaBADH uses NADP+ with the same efficiency as NAD+ | Pseudomonas aeruginosa | |
NADP+ | BADH uses NADP+ with the same efficiency as NAD+ | Pseudomonas aeruginosa |