Cloned (Comment) | Organism |
---|---|
in Escherichia coli strain BL21 (DE3) | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Oryza sativa | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
determined by gel electrophoresis | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminopropionaldehyde + NAD+ + H2O | Oryza sativa | - |
3-aminopropionate + NADH + H+ | - |
? | |
4-aminobutyraldehyde + NAD+ + H2O | Oryza sativa | - |
4-aminobutyrate + NADH + H+ | - |
? | |
betaine aldehyde + NAD+ + H2O | Oryza sativa | belongs to the NAD-dependent dehydrogenase family, characterized by the typical aldehyde substrate binding domain | betaine + NADH + H+ | - |
? | |
additional information | Oryza sativa | 2-acetyl-1-pyrroline, the presence of a dominant Badh2 allele encoding betaine aldehyde dehydrogenase inhibits the synthesis of 2-acetyl-1-pyrroline, a potent flavor component in rice fragrance. By contrast, the recessive allele badh2-E7, induces 2-acetyl-1-pyrroline formation | ? | - |
? | |
additional information | Oryza sativa | 2-acetyl-1-pyrroline; the presence of a dominant Badh2 allele encoding betaine aldehyde dehydrogenase inhibits the synthesis of 2-acetyl-1-pyrroline, a potent flavor component in rice fragrance. By contrast, the recessive allele, badh2-E2, induces 2-acetyl-1-pyrroline formation | ? | - |
? | |
additional information | Oryza sativa | dominant Badh2 allele encoding betaine aldehyde dehydrogenase inhibits the synthesis of 2-acetyl-1-pyrroline, a potent flavor component in rice fragrance. Because the absence of BADH2 protein results in fragrance, this suggests that Badh2 is not directly involved in biosynthesis. Alternative possibilities to explain the effect of BADH2 are that the BADH2 enzyme is involved in a competing pathway in which one of the 2-acetyl-1-pyrroline precursors serves as a BADH2 substrate or that BADH2 participates in 2-acetyl-1-pyrroline catabolism. The intact 503 amino acid BADH2 encoded by the complete Badh2 gene inhibits 2-acetyl-1-pyrroline biosynthesis by converting 4-aminobutyraldehyde to gamma-aminobutyric acid, whereas the absence of BADH2 due to nonfunctional badh2 alleles results in AB-ald accumulation and thus turns on the pathway toward 2-acetyl-1-pyrroline biosynthesis. | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | B3VMC0 | - |
- |
Oryza sativa | B3VMC1 | - |
- |
Oryza sativa | B3VMC2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification Attention: controlled vocabulary for this datafield | commentary | Oryza sativa |
Purification (Comment) | Organism |
---|---|
using nickel-nitrilotriacetic acid agarose affinity chromatography columns after expression in E. coli | Oryza sativa |
using nickel-nitrilotriacetic acid agarose affinity chromatography columns after expression in Escherichia coli | Oryza sativa |
using nickel-nitrilotriacetic acid agarose after cloning and expression in Escherichia coli | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plant | found in all plant tissues excepting roots | Oryza sativa | - |
plant | found in all plant tissues excepting roots, and the transcript is detected at higher abundance in young, healthy leaves than in other tissues | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Determination of aldehyde dehydrogenase activity of purified intact BADH2 and partial BADH2 using various aldehyde substrates (1 mM betaine aldehyde, 50 microM 4-aminobutyraldehyde, and 50 microM 3-aminopropionaldehyde). Enzymatic activities were spectrophotometrically assayed by A340 at pH 8.0 at intervals of 0, 10, 20, 30, and 60 min after the initiation of reactions. The intact BADH2 showed high betaine aldehyde dehydrogenase activity, with a rapid increase in A340 within the first 10 min after the enzymatic reaction. In addition, intact BADH2 also showed strong 4-aminobutyraldehyde and 3-aminopropionaldehyde dehydrogenase activities. | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminopropionaldehyde + NAD+ + H2O | - |
Oryza sativa | 3-aminopropionate + NADH + H+ | - |
? | |
4-aminobutyraldehyde + NAD+ + H2O | - |
Oryza sativa | 4-aminobutyrate + NADH + H+ | - |
? | |
betaine aldehyde + NAD+ + H2O | belongs to the NAD-dependent dehydrogenase family, characterized by the typical aldehyde substrate binding domain | Oryza sativa | betaine + NADH + H+ | - |
? | |
additional information | 2-acetyl-1-pyrroline, the presence of a dominant Badh2 allele encoding betaine aldehyde dehydrogenase inhibits the synthesis of 2-acetyl-1-pyrroline, a potent flavor component in rice fragrance. By contrast, the recessive allele badh2-E7, induces 2-acetyl-1-pyrroline formation | Oryza sativa | ? | - |
? | |
additional information | 2-acetyl-1-pyrroline; the presence of a dominant Badh2 allele encoding betaine aldehyde dehydrogenase inhibits the synthesis of 2-acetyl-1-pyrroline, a potent flavor component in rice fragrance. By contrast, the recessive allele, badh2-E2, induces 2-acetyl-1-pyrroline formation | Oryza sativa | ? | - |
? | |
additional information | dominant Badh2 allele encoding betaine aldehyde dehydrogenase inhibits the synthesis of 2-acetyl-1-pyrroline, a potent flavor component in rice fragrance. Because the absence of BADH2 protein results in fragrance, this suggests that Badh2 is not directly involved in biosynthesis. Alternative possibilities to explain the effect of BADH2 are that the BADH2 enzyme is involved in a competing pathway in which one of the 2-acetyl-1-pyrroline precursors serves as a BADH2 substrate or that BADH2 participates in 2-acetyl-1-pyrroline catabolism. The intact 503 amino acid BADH2 encoded by the complete Badh2 gene inhibits 2-acetyl-1-pyrroline biosynthesis by converting 4-aminobutyraldehyde to gamma-aminobutyric acid, whereas the absence of BADH2 due to nonfunctional badh2 alleles results in AB-ald accumulation and thus turns on the pathway toward 2-acetyl-1-pyrroline biosynthesis. | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | - |
Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
BADH2 | - |
Oryza sativa |
badh2-E2 | recessive allele, truncated form, with a 7-bp deletion in exon 2 (82 residues) | Oryza sativa |
badh2-E7 | recessive allele, truncated form (251 amino acid polypeptide) | Oryza sativa |
betaine aldehyde dehydrogenase | - |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Oryza sativa | |
NAD+ | The BADH2 enzyme is predicted to contain three domains: NAD binding, substrate binding, and oligomerization domain | Oryza sativa |