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Literature summary for 1.2.1.8 extracted from

  • Cui, X.; Wang, Y.; Guo, J.
    Osmotic regulation of betaine content in Leymus chinensis under saline-alkali stress and cloning and expression of betaine aldehyde dehydrogenase (BADH) gene (2008), Chem. Res. Chin. Univ., 24, 204-209.
No PubMed abstract available
Search for more literature for 1.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
propan-2-yl 1-thio-beta-D-galactopyranoside for the expressed protein, final concentration of 1 mmol in culture solution Leymus chinensis

Application

Application Comment Organism
agriculture potential: the BADH gene can be cloned from Leymus chinensis under stress treatment, which indicates that its expression and regulation may play an important role in stress tolerance. Therefore, it can be transformed into other plants to obtain transgenic species with a high saline-alkali tolerance by biotechnology; as a result, it can speed up the recovering and rebuilding of saline-alkaline grassland Leymus chinensis

Cloned(Commentary)

Cloned (Comment) Organism
the BADH gene of Leymus chinensis is cloned by RT-PCR and RACE technology and is designated as LcBADH. The cDNA sequence of LcBADH is 1774 bp including the open reading frame of 1521 bp (coding 506 amino acids). The vector of prokaryotic expression is constructed by inserting the LcBADH gene fragment into pET30a(+) and transformed into Escherichia coli BL21(DE3) Leymus chinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome putative Leymus chinensis 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- treatment of seedlings after germinating with 6.25-200 mmol/l Leymus chinensis
Na+ treatment of seedlings after germinating with 18.75-400 mmol/l Leymus chinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56780
-
 mass of expressed protein (LcBADH) determinating by SDS-PAGE Leymus chinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Leymus chinensis
-
 betaine + NADH + 2 H+ accumulation of betaine is a strategy of plants to survive drought, salinity, and extreme temperatures ?

Organism

Organism UniProt Comment Textmining
Leymus chinensis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
betaine aldehyde + NAD+ + H2O = betaine + NADH + 2 H+ catalyzes the last step of the two-step oxidation Leymus chinensis
a

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Leymus chinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00826
-
 specific activity of expressed protein after 3 h IPTG induction. After disruption of the cells by ultrasonication, supernatant is used to assay the enzyme activity. Reaction is started by adding 0.05 ml of 10 mmol/l betaine aldehyde chloride at 30°C and lasted for 30 min. Specific activity is detected in crude enzyme extracts. BADH activity from induced bacteria is 75.3% higher than in the control. Leymus chinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O
-
 Leymus chinensis betaine + NADH + 2 H+ accumulation of betaine is a strategy of plants to survive drought, salinity, and extreme temperatures ?

Synonyms

Synonyms Comment Organism
BADH
-
 Leymus chinensis
betaine aldehyde dehydrogenase
-
 Leymus chinensis
LcBADH
-
 Leymus chinensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 assay at Leymus chinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.6
-
 assay at Leymus chinensis

pH Range

pH Minimum pH Maximum Comment Organism
7.8 10.1 assay at Leymus chinensis