Application | Comment | Organism |
---|---|---|
medicine | Pseudomonas aeruginosa is an important pathogen, glycine betaine could play an important physiological role under the conditions present in the infection sites | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
the gene betB, encoding for Pseudomonas aeruginosa betaine-aldehyde dehydrogenase, is cloned into a pCAL-n vector for sequencing and protein expression in Escherichia coli | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53330 | - |
monomer, amino acid sequence | Pseudomonas aeruginosa |
61000 | - |
SDS-PAGE, Coomassie blue staining and Western blot | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | Pseudomonas aeruginosa | - |
glycine betaine + NADH + 2 H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HTJ1 | - |
- |
Purification (Comment) | Organism |
---|---|
purified on Q-Sepharose fast flow and 2', 5'-ADP-Sepharose columns, 28 mg of pure protein per liter of culture are yielded | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.006 | - |
culture conditions, 11.1 mM glucose and 15 mM NH4Cl | Pseudomonas aeruginosa |
0.012 | - |
culture conditions, 11.1 mM glucose, 15 mM NH4Cl and 0.4 M NaCl | Pseudomonas aeruginosa |
0.211 | - |
culture conditions, 11.1 mM glucose, 20 mM choline and 0.4 M NaCl | Pseudomonas aeruginosa |
0.215 | - |
culture conditions, 11.1 mM glucose and 20 mM choline | Pseudomonas aeruginosa |
0.492 | - |
culture conditions, 20 mM choline and 0.4 M NaCl | Pseudomonas aeruginosa |
0.571 | - |
culture conditions, 20 mM choline | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | - |
Pseudomonas aeruginosa | glycine betaine + NADH + 2 H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 x 61000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
BADH | - |
Pseudomonas aeruginosa |
betaine aldehyde:NAD(P)+ oxidoreductase | - |
Pseudomonas aeruginosa |
betaine-aldehyde dehydrogenase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |