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Literature summary for 1.2.1.79 extracted from

  • Ito, S.; Osanai, T.
    Unconventional biochemical regulation of the oxidative pentose phosphate pathway in the model cyanobacterium Synechocystis sp. PCC 6803 (2020), Biochem. J., 477, 1309-1321 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate about 35% activation at 5 mM Synechocystis sp. PCC 6803
citrate about 25% activation at 5 mM Synechocystis sp. PCC 6803
oxaloacetate about 15% activation at 5 mM Synechocystis sp. PCC 6803

Cloned(Commentary)

Cloned (Comment) Organism
gene gabD, recombinant expression of GST-tagged enzyme in Escherichia coli strain DH5alpha Synechocystis sp. PCC 6803

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by citrate Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Synechocystis sp. PCC 6803
0.01
-
succinate semialdehyde pH 9.7, 45°C, recombinant detagged enzyme Synechocystis sp. PCC 6803
0.058
-
NADP+ pH 9.7, 45°C, recombinant detagged enzyme Synechocystis sp. PCC 6803

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NADP+ + H2O Synechocystis sp. PCC 6803
-
succinate + NADPH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 Q55585
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography and ultrafiltration, followed by tag cleavage through PreScission protease in a batch and the protease resin is eliminated by centrifugation Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NADP+ + H2O
-
Synechocystis sp. PCC 6803 succinate + NADPH + 2 H+
-
?

Synonyms

Synonyms Comment Organism
gabD
-
Synechocystis sp. PCC 6803
slr0370 locus name Synechocystis sp. PCC 6803
SSADH
-
Synechocystis sp. PCC 6803
succinic semialdehyde dehydrogenase
-
Synechocystis sp. PCC 6803
SySSADH
-
Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
recombinant enzyme Synechocystis sp. PCC 6803

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.38
-
succinate semialdehyde pH 9.7, 45°C, recombinant detagged enzyme Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.7
-
recombinant enzyme Synechocystis sp. PCC 6803

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Synechocystis sp. PCC 6803

General Information

General Information Comment Organism
metabolism SySSADH catalyzes one of the NAD(P)H generating reactions in the oxidative TCA cycle. The oxidative TCA cycle is a pathway with low efficiency in NADPH generation in Synechocystis sp. 6803. Similar to isocitrate dehydrogenase from Synechocystis sp. 6803, SySSADH specifically catalyzes the NADPH-generating reaction and is not inhibited by citrate. To avoid NADPH overproduction, the oxidative pentose phosphate (OPP) pathway dehydrogenase activity is repressed when the flow of the oxidative TCA cycle increases in Synechocystis sp. 6803. Metabolic map around the OPP pathway, overview Synechocystis sp. PCC 6803
physiological function succinic semialdehyde dehydrogenase (SSADH) from Synechocystis 6803 (SySSADH) catalyzes one of the NAD(P)H generating reactions in the oxidative TCA cycle. Similar to isocitrate dehydrogenase from Synechocystis sp. 6803, SySSADH specifically catalyzes the NADPH-generating reaction and is not inhibited by citrate. The activity of SySSADH is lower than that of other bacterial SSADHs Synechocystis sp. PCC 6803

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23.8
-
NADP+ pH 9.7, 45°C, recombinant detagged enzyme Synechocystis sp. PCC 6803
138
-
succinate semialdehyde pH 9.7, 45°C, recombinant detagged enzyme Synechocystis sp. PCC 6803