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Literature summary for 1.2.1.79 extracted from

  • Kim, H.T.; Khang, T.U.; Baritugo, K.A.; Hyun, S.M.; Kang, K.H.; Jung, S.H.; Song, B.K.; Park, K.; Oh, M.K.; Kim, G.B.; Kim, H.U.; Lee, S.Y.; Park, S.J.; Joo, J.C.
    Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical (2019), Metab. Eng., 51, 99-109 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gabD, recombinant expression in Corynebacterium glutamicum strain KCTC 1857, coexpression with 5-aminovalerate transaminase (davT, EC 2.6.1.48) from Pseudomonas putida, 4-aminobutyrate-2-oxoglutarate transaminase (gabT, EC 2.6.1.19) from Corynebacterium glutamicum, and glutarate semialdehyde dehydrogenase (gabD, EC 1.2.1.20) from Pseudomonas putida, as well as N-terminal His6-tagged lysine 2-monooxygenase (davB, EC 1.13.12.2) from Pseudomonas putida Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical, by co-expression of Pseudomonas putida davT, davB, and davD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively, in Corynebacterium glutamicum. Method optimization and evaluation. The glutaric acid biosynthesis pathway constructed in recombinant Corynebacterium glutamicum is engineered by examining strong synthetic promoters H30 and H36, Corynebacterium glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag to improve the production of glutaric acid. The use of N-terminal His6-tagged DavB is most suitable for the production of glutaric acid from glucose. Fed-batch fermentation of the final engineered Corynebacterium glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His6-tag at N-terminus can produce 24.5 g/l of glutaric acid with low accumulation of L-lysine (1.7 g/l), wherein 5-aminovaleric acid (5-AVA) ccumulation is not observed during fermentation. Metabolically engineered Corynebacterium glutamicum strain KCTC H30_GA-2 (engineered strain KCTC 1857) is able for catalysis of the biosynthesis of glutaric acid from glucose. Method optimization and evaluation, overview Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NADP+ + H2O Corynebacterium glutamicum
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succinate + NADPH + 2 H+
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?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum A0A1Q6BLU5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NADP+ + H2O
-
Corynebacterium glutamicum succinate + NADPH + 2 H+
-
?

Synonyms

Synonyms Comment Organism
gabD
-
Corynebacterium glutamicum
NADP-dependent succinic semialdehyde dehydrogenase
-
Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Corynebacterium glutamicum

General Information

General Information Comment Organism
physiological function production of glutaric acid depends on the expression of native gabT (EC 2.6.1.48) and gabD of Corynebacterium glutamicum, or on heterologous expression of davT (EC 2.6.1.48) and davD (EC 1.2.1.20) from Pseudomonas putida encoding 5-aminovalerate aminotransferase, and glutarate semialdehyde, respectively Corynebacterium glutamicum