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Literature summary for 1.2.1.78 extracted from

  • Kiyohara, H.; Nagao, K.; Yano, K.
    Isolation and some properties of NAD-linked 2-carboxybenzaldehyde dehydrogenase in Alcaligenes faecalis AFK 2 grown on phenanthrene (1981), J. Gen. Appl. Microbiol., 27, 443-455.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-naphthoaldehyde 0.2 mM, 34.6% inhibition Alcaligenes faecalis
4-carboxybenzaldehyde 0.2 mM, 38.5% inhibition Alcaligenes faecalis
acetaldehyde 0.2 mM, 34.6% inhibition Alcaligenes faecalis
Ag+ 1 mM, complete inhibition Alcaligenes faecalis
Co2+ 1 mM, 40.2% inhibition Alcaligenes faecalis
Cu2+ 1 mM, complete inhibition Alcaligenes faecalis
Hg2+ 1 mM, 17.5% inhibition Alcaligenes faecalis
iso-phthalaldehyde 0.2 mM, 23.8% inhibition Alcaligenes faecalis
o-nitrobenzaldehyde 0.2 mM, 58.1% inhibition Alcaligenes faecalis
o-phthalaldehyde 0.2 mM, 91.4% inhibition Alcaligenes faecalis
p-chloromercuribenzoate 1 mM, complete inhibition Alcaligenes faecalis
p-dimethylamino benzaldehyde 0.2 mM, 32.7% inhibition Alcaligenes faecalis
propionaldehyde 0.2 mM, 31.7% inhibition Alcaligenes faecalis
Protocatechualdehyde 0.2 mM, 23.1% inhibition Alcaligenes faecalis
Salicylaldehyde 0.2 mM, 23.1% inhibition Alcaligenes faecalis
SDS 1 mM, 44.3% inhibition Alcaligenes faecalis
vertraldehyde 0.2 mM, 13.5% inhibition Alcaligenes faecalis
Zn2+ 1 mM, 71.7% inhibition Alcaligenes faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
2-carboxybenzaldehyde pH 8.0, 30°C Alcaligenes faecalis
0.14
-
NAD+ pH 8.0, 30°C Alcaligenes faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
4 * 41000, SDS-PAGE Alcaligenes faecalis
158000
-
gel filtration Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-carboxybenzaldehyde + NAD+ + H2O Alcaligenes faecalis the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene o-phthalic acid + NADH + H+
-
?
2-carboxybenzaldehyde + NAD+ + H2O Alcaligenes faecalis AFK2 the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene o-phthalic acid + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis AFK2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Alcaligenes faecalis

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:phenanthrene-grown cell
-
Alcaligenes faecalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
130.6
-
-
Alcaligenes faecalis

Storage Stability

Storage Stability Organism
4°C, activity in phosphate buffer is labile, 1 mM DTT stabilizes Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-carboxybenzaldehyde + NAD+ + H2O the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene Alcaligenes faecalis o-phthalic acid + NADH + H+
-
?
2-carboxybenzaldehyde + NAD+ + H2O high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor Alcaligenes faecalis o-phthalic acid + NADH + H+
-
?
2-carboxybenzaldehyde + NAD+ + H2O the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene Alcaligenes faecalis AFK2 o-phthalic acid + NADH + H+
-
?
2-carboxybenzaldehyde + NAD+ + H2O high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor Alcaligenes faecalis AFK2 o-phthalic acid + NADH + H+
-
?
additional information no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde Alcaligenes faecalis ?
-
?
additional information no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde Alcaligenes faecalis AFK2 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Alcaligenes faecalis

Synonyms

Synonyms Comment Organism
2CBAL dehydrogenase
-
Alcaligenes faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Alcaligenes faecalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
10 min, no loss of activity below 30°C Alcaligenes faecalis
40
-
10 min, 25% loss of activity Alcaligenes faecalis
50
-
10 min, 94% loss of activity Alcaligenes faecalis
60
-
10 min, complete loss of activity Alcaligenes faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Alcaligenes faecalis

pH Range

pH Minimum pH Maximum Comment Organism
7 8.6 pH 7.0: about 60% of maximal activity, pH 8.6: about 70% of maximal activity Alcaligenes faecalis

Cofactor

Cofactor Comment Organism Structure
NAD+ high specificity for NAD+ as electron acceptor Alcaligenes faecalis