Cloned (Comment) | Organism |
---|---|
gene safD, DNA and amino acid sequence determination and analysis, genetic organization | Neptuniibacter caesariensis |
General Stability | Organism |
---|---|
SafD is stable in Tris-HCl buffer, pH 9.0 | Neptuniibacter caesariensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | low activity an inhibition at higher concentration | Neptuniibacter caesariensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
NAD+ | pH 9.0, 22°C | Neptuniibacter caesariensis | |
0.49 | - |
Sulfoacetaldehyde | pH 9.0, 22°C | Neptuniibacter caesariensis | |
4.5 | - |
NADP+ | pH 9.0, 22°C | Neptuniibacter caesariensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Neptuniibacter caesariensis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Neptuniibacter caesariensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49100 | - |
4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation | Neptuniibacter caesariensis |
50000 | - |
4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation | Neptuniibacter caesariensis |
190000 | - |
gel filtration | Neptuniibacter caesariensis |
196500 | - |
sequence calculation | Neptuniibacter caesariensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-sulfoacetaldehyde + H2O + NAD+ | Neptuniibacter caesariensis | - |
sulfoacetate + NADH + H+ | - |
? | |
additional information | Neptuniibacter caesariensis | the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neptuniibacter caesariensis | - |
formerly Oceanospirillum sp., CCUG 52065, a marine gammaproteobacterium, strain MED92, gene safD encoding an inducible enzyme | - |
Purification (Comment) | Organism |
---|---|
native enzyme 610fold to homogeneity by anion exchange and hydrophobic interaction chromatography | Neptuniibacter caesariensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-sulfoacetaldehyde + H2O + NAD(P)+ | the enzyme is specific for sulfoacetaldehyde, NAD+ is the highly preferred cofactor to NADP+ | Neptuniibacter caesariensis | sulfoacetate + NAD(P)H + H+ | - |
? | |
2-sulfoacetaldehyde + H2O + NAD+ | - |
Neptuniibacter caesariensis | sulfoacetate + NADH + H+ | - |
? | |
additional information | the organism assimilates taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC transporter, taurine:pyruvate aminotransferase, a sulfoacetaldehyde dehydrogenase, and exporter(s) of sulfoacetate, overview | Neptuniibacter caesariensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, SDS-PAGE, 4 * 49100, sequence calculation | Neptuniibacter caesariensis |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the NAD-dependent aldehyde dehydrogenase superfamily | Neptuniibacter caesariensis |
SafD | - |
Neptuniibacter caesariensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Neptuniibacter caesariensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Neptuniibacter caesariensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 10.5 | 50% of maximal activity at pH 7.2, 70% at pH 8.0, and 80% at pH 9.5-10.5 | Neptuniibacter caesariensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | highly preferred cofactor | Neptuniibacter caesariensis | |
NADP+ | low activity and inhibition at higher concentration | Neptuniibacter caesariensis |