Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, increase in activity | Streptomyces cattleya |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chloroacetaldehyde | 0.25 mM, time-dependent inactivation | Streptomyces cattleya | |
Cu2+ | 1 mM, strong inhibition | Streptomyces cattleya | |
iodoacetamide | 0.05 mM, strong inhibition | Streptomyces cattleya | |
Mg2+ | 1 mM, 80% loss of activity, possibly due to a decreased dissociation rate of NADH from the enzyme | Streptomyces cattleya |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
fluoroacetaldehyde | - |
Streptomyces cattleya | |
0.15 | - |
glycoaldehyde | - |
Streptomyces cattleya | |
0.32 | - |
benzaldehyde | - |
Streptomyces cattleya | |
0.81 | - |
acetaldehyde | - |
Streptomyces cattleya |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Streptomyces cattleya |
200000 | - |
gel filtration | Streptomyces cattleya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fluroracetaldehyde + NAD+ + H2O | Streptomyces cattleya | involved in fluorometabolite biosynthesis | fluoroacetate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cattleya | - |
NRRL 8057 | - |
Streptomyces cattleya NRRL 8057 | - |
NRRL 8057 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, anion-exchange, 5'-AMP-agarose | Streptomyces cattleya |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.889 | - |
- |
Streptomyces cattleya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | 40% of activity with fluoroacetaldehyde | Streptomyces cattleya | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | 40% of activity with fluoroacetaldehyde | Streptomyces cattleya NRRL 8057 | acetate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 65% of activity with fluoroacetaldehyde | Streptomyces cattleya | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 65% of activity with fluoroacetaldehyde | Streptomyces cattleya NRRL 8057 | benzoate + NADH + H+ | - |
? | |
chloroacetaldehyde + NAD+ + H2O | 80% of activity with fluoroacetaldehyde, inactivation upon extended incubation | Streptomyces cattleya | chloroacetate + NADH + H+ | - |
? | |
chloroacetaldehyde + NAD+ + H2O | 80% of activity with fluoroacetaldehyde, inactivation upon extended incubation | Streptomyces cattleya NRRL 8057 | chloroacetate + NADH + H+ | - |
? | |
DL-glyceraldehyde + NAD+ + H2O | 10% of activity with fluoroacetaldehyde | Streptomyces cattleya | DL-glycerate + NADH + H+ | - |
? | |
fluroracetaldehyde + NAD+ + H2O | - |
Streptomyces cattleya | fluoroacetate + NADH + H+ | - |
? | |
fluroracetaldehyde + NAD+ + H2O | - |
Streptomyces cattleya NRRL 8057 | fluoroacetate + NADH + H+ | - |
? | |
fluroracetaldehyde + NAD+ + H2O | involved in fluorometabolite biosynthesis | Streptomyces cattleya | fluoroacetate + NADH | - |
? | |
glycoaldehyde + NAD+ + H2O | 150% of activity with fluoroacetaldehyde | Streptomyces cattleya | glycolate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | 28% of activity with fluoroacetaldehyde | Streptomyces cattleya | propionate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | 28% of activity with fluoroacetaldehyde | Streptomyces cattleya NRRL 8057 | propionate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Streptomyces cattleya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Streptomyces cattleya |