Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 40% inhibition at 1 mM | Pseudomonas sp. | |
2,2'-dipyridyl | 50% inhibition at 1 mM | Pseudomonas sp. | |
2-naphthaldehyde | substrate inhibition | Pseudomonas sp. | |
3-Chlorobenzaldehyde | substrate inhibition | Pseudomonas sp. | |
3-Hydroxybenzaldehyde | substrate inhibition | Pseudomonas sp. | |
4-Chlorobenzaldehyde | substrate inhibition | Pseudomonas sp. | |
4-nitrobenzaldehyde | substrate inhibition | Pseudomonas sp. | |
benzaldehyde | substrate inhibition | Pseudomonas sp. | |
EDTA | 50% inhibition at 50 mM | Pseudomonas sp. | |
EGTA | 60% inhibition at 50 mM | Pseudomonas sp. | |
Na+ | inhibitory at higher concentration | Pseudomonas sp. | |
Salicylaldehyde | substrate inhibition, 86% and 70% decrease in activity at 0.05 mM and 0.1 mM, respectively | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics for NAD+ | Pseudomonas sp. | |
0.001 | - |
benzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.0021 | - |
3-Hydroxybenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.0035 | - |
2-naphthaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.0039 | - |
3-Chlorobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.0045 | - |
Salicylaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.0064 | - |
4-Chlorobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.011 | - |
4-nitrobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.052 | - |
NAD+ | pH 8.0, temperature not specified in the publication | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme contains 1.8 mol/mol of enzyme | Pseudomonas sp. | |
Fe2+ | the enzyme contains 0.4 mol/mol of enzyme | Pseudomonas sp. | |
K+ | 44% activating at 200 mM | Pseudomonas sp. | |
Mg2+ | the enzyme contains 0.3 mol/mol of enzyme | Pseudomonas sp. | |
additional information | external addition of Ca2+, Mg2+, Fe2+ and Fe3+ at 10 mM, either as sulfate or chloride salts, fail to show an increase in the enzyme activity | Pseudomonas sp. | |
Zn2+ | the enzyme contains 0.2 mol/mol of enzyme | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
salicylaldehyde + NAD+ + H2O | Pseudomonas sp. | - |
salicylate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 28fold from cellfree extract by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.3 | - |
purified native enzyme, pH 8.0, temperature not specified in the publication | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthaldehyde + NAD+ + H2O | low activity | Pseudomonas sp. | 1-naphthoate + NADH + 2 H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | very low activity | Pseudomonas sp. | 2-carboxybenzoate + NADH + 2 H+ | - |
? | |
2-chlorobenzaldehyde + NAD+ + H2O | low activity | Pseudomonas sp. | 2-chlorobenzoate + NADH + 2 H+ | - |
? | |
2-methoxybenzaldehyde + NAD+ + H2O | low activity | Pseudomonas sp. | 2-methoxybenzoate + NADH + 2 H+ | - |
? | |
2-naphthaldehyde + NAD+ + H2O | moderate activity | Pseudomonas sp. | 2-naphthoate + NADH + 2 H+ | - |
? | |
2-nitrobenzaldehyde + NAD+ + H2O | low activity | Pseudomonas sp. | 2-nitrobenzoate + NADH + 2 H+ | - |
? | |
3-carboxybenzaldehyde + NAD+ + H2O | very low activity | Pseudomonas sp. | 3-carboxybenzoate + NADH + 2 H+ | - |
? | |
3-chlorobenzaldehyde + NAD+ + H2O | moderate activity | Pseudomonas sp. | 3-chlorobenzoate + NADH + 2 H+ | - |
? | |
3-hydroxybenzaldehyde + NAD+ + H2O | low to moderate activity | Pseudomonas sp. | 3-hydroxybenzoate + NADH + 2 H+ | - |
? | |
3-methoxybenzaldehyde + NAD+ + H2O | moderate activity | Pseudomonas sp. | 3-methoxybenzoate + NADH + 2 H+ | - |
? | |
3-nitrobenzaldehyde + NAD+ + H2O | low activity | Pseudomonas sp. | 3-nitrobenzoate + NADH + 2 H+ | - |
? | |
4-carboxybenzaldehyde + NAD+ + H2O | very low activity | Pseudomonas sp. | 4-carboxybenzoate + NADH + 2 H+ | - |
? | |
4-chlorobenzaldehyde + NAD+ + H2O | high activity | Pseudomonas sp. | 4-chlorobenzoate + NADH + 2 H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | low activity | Pseudomonas sp. | 4-hydroxybenzoate + NADH + 2 H+ | - |
? | |
4-methoxybenzaldehyde + NAD+ + H2O | low activity | Pseudomonas sp. | 4-methoxybenzoate + NADH + 2 H+ | - |
? | |
4-nitrobenzaldehyde + NAD+ + H2O | moderate activity | Pseudomonas sp. | 4-nitrobenzoate + NADH + 2 H+ | - |
? | |
acetaldehyde + NAD+ + H2O | very low activity | Pseudomonas sp. | acetate + NADH + 2 H+ | - |
? | |
benzaldehyde + NAD+ + H2O | moderate to high activity | Pseudomonas sp. | benzoate + NADH + 2 H+ | - |
? | |
butyraldehyde + NAD+ + H2O | low activity | Pseudomonas sp. | butyrate + NADH + 2 H+ | - |
? | |
formaldehyde + NAD+ + H2O | very low activity | Pseudomonas sp. | formate + NADH + 2 H+ | - |
? | |
glutaraldehyde + NAD+ + H2O | very low activity | Pseudomonas sp. | glutarate + NADH + 2 H+ | - |
? | |
isovaleraldehyde + NAD+ + H2O | very low activity | Pseudomonas sp. | isovalerate + NADH + 2 H+ | - |
? | |
additional information | the enzyme shows maximum activity on salicylaldehyde and very poor activity on aliphatic aldehydes. The preferences of substrate (derivatives of benzaldehyde) for enzyme SALDH are dependent on nature, position and group of substituents. Enzyme SALDH prefers: (1) hydroxyl derivative at ortho and meta position, (2) chloro and nitro derivative at meta and para position, and (3) methoxy at meta position. The carboxyl group substitution is poorly preferred. Enzyme SALDH also shows activity on di-aromatic aldehydes. Substrate specificity, overview | Pseudomonas sp. | ? | - |
? | |
propionaldehyde + NAD+ + H2O | very low activity | Pseudomonas sp. | propionate + NADH + 2 H+ | - |
? | |
salicylaldehyde + NAD+ + H2O | - |
Pseudomonas sp. | salicylate + NADH + 2 H+ | - |
? | |
salicylaldehyde + NAD+ + H2O | best substrate | Pseudomonas sp. | salicylate + NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 50000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent salicylaldehyde dehydrogenase | - |
Pseudomonas sp. |
sALDH | - |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
3-Hydroxybenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
26.4 | - |
benzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
59 | - |
4-nitrobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
65 | - |
3-Chlorobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
93.6 | - |
Salicylaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
96 | - |
2-naphthaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
98 | - |
4-Chlorobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme SALDH shows poor activity with NADP+ | Pseudomonas sp. | |
NAD+ | dependent on | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
3-Chlorobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.0773 | - |
4-Chlorobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.126 | - |
4-nitrobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.136 | - |
2-naphthaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.185 | - |
Salicylaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
0.306 | - |
benzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
1.364 | - |
3-Hydroxybenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the oxidation of salicylaldehyde to salicylate in the carbaryl-degrading Pseudomonas sp. strain C6 | Pseudomonas sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6000 | - |
4-nitrobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
8800 | - |
3-Hydroxybenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
12400 | - |
4-Chlorobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
21500 | - |
3-Chlorobenzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
23000 | - |
Salicylaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
26000 | - |
benzaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. | |
29000 | - |
2-naphthaldehyde | pH 8.0, temperature not specified in the publication | Pseudomonas sp. |