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Literature summary for 1.2.1.60 extracted from

  • Alonso, J.M.; Garrido-Pertierra, A.
    Kinetic properties of 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase from Escherichia coli (1986), Biochimie, 68, 731-737.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP competitive inhibition, Ki: 0.088 mM Escherichia coli
NADH competitive inhibition, Ki: 0.025 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
Escherichia coli
0.029
-
NAD+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Escherichia coli involved in the meta-cleavage pathway of 4-hydroxyphenylacetate 5-carboxymethyl-2-hydroxymuconate + NADH
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Escherichia coli B / ATCC 11303 involved in the meta-cleavage pathway of 4-hydroxyphenylacetate 5-carboxymethyl-2-hydroxymuconate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ involved in the meta-cleavage pathway of 4-hydroxyphenylacetate Escherichia coli 5-carboxymethyl-2-hydroxymuconate + NADH
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ involved in the meta-cleavage pathway of 4-hydroxyphenylacetate Escherichia coli B / ATCC 11303 5-carboxymethyl-2-hydroxymuconate + NADH
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
stable for 30 min Escherichia coli
42
-
loss of activity after 10 min Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli