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Literature summary for 1.2.1.60 extracted from

  • Alonso, J.M.; Garrido-Pertierra, A.
    Carboxymethylhydroxymuconic semialdehyde dehydrogenase in the 4-hydroxyphenylacetate catabolic pathway of Pseudomonas putida (1986), Biochem. Cell Biol., 64, 1288-1293.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
NADH competitive inhibition with respect to NAD+, Ki: 0.027 mM Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
Pseudomonas putida
0.02
-
NAD+
-
Pseudomonas putida
0.048
-
NAD+
-
Pseudomonas putida
1.2
-
NADP+
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52500
-
3 * 52500, SDS-PAGE Pseudomonas putida
200000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Pseudomonas putida involved in the meta-cleavage pathway of 4-hydroxyphenylacetate 5-carboxymethyl-2-hydroxymuconate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
frozen, 12 months Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ involved in the meta-cleavage pathway of 4-hydroxyphenylacetate Pseudomonas putida 5-carboxymethyl-2-hydroxymuconate + NADH
-
?
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+ 18% of the activity compared to reduction with NAD+ Pseudomonas putida 5-carboxymethyl-2-hydroxymuconate + NADPH
-
?

Subunits

Subunits Comment Organism
tetramer 3 * 52500, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable for 30 min Pseudomonas putida
47
-
complete loss of activity after 20 min Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
5-carboxymethyl-2-hydroxymuconate semialdehyde
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ strict dependency for NAD+ reduction Pseudomonas putida
NADP+ 82% lower activity compared to NAD+ Pseudomonas putida