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Literature summary for 1.2.1.5 extracted from

  • Gonzalez-Segura, L.; Ho, K.K.; Perez-Miller, S.; Weiner, H.; Hurley, T.D.
    Catalytic contribution of threonine 244 in human ALDH2 (2013), Chem. Biol. Interact., 202, 32-40.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
the apoenzyme is crystallized by using 100 mM ACES, pH 6.4, 100-200 mM guanidine-HCl, 1-10 mM MgCl2 and 16–17% (w/v) PEG 6000, the mutant T244A in complex with NAD is crystallized by using 100 mM ACES, pH 6.4, 100 mM guanidine–HCl, 10 mM MgCl2 and 18% (w/v) PEG 6000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
T244A the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme Homo sapiens
T244G the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme Homo sapiens
T244S the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme Homo sapiens
T244V the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 Chloroacetaldehyde mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.039
-
 Chloroacetaldehyde wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.048
-
 NAD+ wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.052
-
 Chloroacetaldehyde mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.094
-
 NAD+ mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.19
-
 NAD+ mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.3
-
 Chloroacetaldehyde mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.39
-
 NAD+ mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.46
-
 Chloroacetaldehyde mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
2.1
-
 NAD+ mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulatory effect at 5 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 4 * 55000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chloroacetaldehyde + NAD+ + H2O Homo sapiens
-
 chloroacetate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O Homo sapiens
-
 propionate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, 5’-AMP Sepharose 4B and 4-hydroxyacetophenone affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chloroacetaldehyde + NAD+ + H2O
-
 Homo sapiens chloroacetate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 55000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ALDH2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.47
-
 NAD+ mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.47
-
 Chloroacetaldehyde mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.63
-
 NAD+ mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
0.63
-
 Chloroacetaldehyde mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
2.5
-
 NAD+ mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
2.5
-
 Chloroacetaldehyde mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
4
-
 NAD+ mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
4
-
 Chloroacetaldehyde mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
7.7
-
 NAD+ wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
7.7
-
 Chloroacetaldehyde wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8
-
 NAD+ mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
3.3
-
 NAD+ mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
5.5
-
 Chloroacetaldehyde mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
6.5
-
 NAD+ mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
12.2
-
 Chloroacetaldehyde mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
13.2
-
 Chloroacetaldehyde mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
33.3
-
 NAD+ mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
158.3
-
 NAD+ wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
183.3
-
 Chloroacetaldehyde mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
200
-
 Chloroacetaldehyde wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens