Application | Comment | Organism |
---|---|---|
degradation | degradation of polyethylene glycols, PEGs | Sphingopyxis macrogoltabida |
Cloned (Comment) | Organism |
---|---|
expression as His-tag fusion protein in Echerichia coli Rosetta (DE3) pLys | Sphingopyxis macrogoltabida |
the pegC gene of Sphingomonas macrogoltabidus is cloned into the pGEM-T and pET23d vectors for expression of nicotinprotein aldehyde dehydrogenase in Escherichia coli Rosetta DE3 pLysS | Sphingopyxis macrogoltabida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
Cu2+ | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
Hg2+ | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
p-chloromercuribenzoate | 50 microM, 80% inhibition | Sphingopyxis macrogoltabida | |
p-mercuribenzoate | 80% inhibition at 0.05 mM | Sphingopyxis macrogoltabida | |
Pb2+ | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
pyrazole | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
trans-4-(N,N-dimethylamino)-cinnamaldehyde | 1 mM, 58% inhibition; 58% inhibition at 1 mM | Sphingopyxis macrogoltabida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.137 | - |
n-dodecanal | 50 mM potassium phosphate buffer pH8.0, 1 mM CaCl2, 36°C | Sphingopyxis macrogoltabida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Sphingopyxis macrogoltabida | |
Ca2+ | stimulating | Sphingopyxis macrogoltabida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46100 | - |
SDS-PAGE, recombinant enzyme | Sphingopyxis macrogoltabida |
46100 | - |
molecular weight of one subunit, determined by gel filtration on a Superdex column and by SDS-PAGE | Sphingopyxis macrogoltabida |
190000 | - |
190000, molecular mass of the native enzyme is estimated by gel filtration, indicating that the enzyme is a homotetramer | Sphingopyxis macrogoltabida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + NAD(P)+ + H2O | Sphingopyxis macrogoltabida | - |
an acid + NAD(P)H + H+ | - |
? | |
an aldehyde + NAD(P)+ + H2O | Sphingopyxis macrogoltabida 103 | - |
an acid + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingopyxis macrogoltabida | - |
- |
- |
Sphingopyxis macrogoltabida 103 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity chromatography | Sphingopyxis macrogoltabida |
on a Ni-NTA column | Sphingopyxis macrogoltabida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Sphingopyxis macrogoltabida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.07 | - |
cell free extract | Sphingopyxis macrogoltabida |
0.49 | - |
after the Ni-NTA purification step | Sphingopyxis macrogoltabida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | acetate + NADPH + H+ | - |
? | |
acetaldehyde + NADP+ + H2O | - |
Sphingopyxis macrogoltabida 103 | acetate + NADPH + H+ | - |
? | |
an aldehyde + NAD(P)+ + H2O | - |
Sphingopyxis macrogoltabida | an acid + NAD(P)H + H+ | - |
? | |
an aldehyde + NAD(P)+ + H2O | - |
Sphingopyxis macrogoltabida 103 | an acid + NAD(P)H + H+ | - |
? | |
formaldehyde + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | formate + NADPH + H+ | - |
? | |
formaldehyde + NADP+ + H2O | - |
Sphingopyxis macrogoltabida 103 | formate + NADPH + H+ | - |
? | |
n-butanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-butanoate + NADPH + H+ | - |
? | |
n-butanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida 103 | n-butanoate + NADPH + H+ | - |
? | |
n-decanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-decanoate + NADPH + H+ | - |
? | |
n-decanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida 103 | n-decanoate + NADPH + H+ | - |
? | |
n-dodecanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-dodecanoate + NADPH + H+ | - |
? | |
n-hexanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-hexanoate + NADPH + H+ | - |
? | |
n-nonanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-nonanoate + NADPH + H+ | - |
? | |
n-octanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-octanoate + NADPH + H+ | - |
? | |
n-pentanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-pentanoate + NADPH + H+ | - |
? | |
n-propanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-propanoate + NADPH + H+ | - |
? | |
n-tetradecanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-tetradecanoate + NADPH + H+ | - |
? | |
n-tridecanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-tridecanoate + NADPH + H+ | - |
? | |
n-undecanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-undecanoate + NADPH + H+ | - |
? | |
polyethylene glycol 1000-aldehyde + NADP+ | - |
Sphingopyxis macrogoltabida | ? + NADPH | - |
? | |
polyethylene glycol 200-aldehyde + NADP+ | - |
Sphingopyxis macrogoltabida | ? + NADPH | - |
? | |
polyethylene glycol 400-aldehyde + NADP+ | - |
Sphingopyxis macrogoltabida | ? + NADPH | - |
? | |
polyethylene glycol 4000-aldehyde + NADP+ | highest activtiy compared to other polyethylene glycolaldehydes | Sphingopyxis macrogoltabida | ? + NADPH | - |
? | |
polyethylene glycol 6000-aldehyde + NADP+ | - |
Sphingopyxis macrogoltabida | ? + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 190000, molecular mass of the native enzyme is estimated by gel filtration, indicating that the enzyme is a homotetramer | Sphingopyxis macrogoltabida |
homotetramer | 4 * 46100, gel filtration | Sphingopyxis macrogoltabida |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase | - |
Sphingopyxis macrogoltabida |
ALDH | - |
Sphingopyxis macrogoltabida |
nicotinprotein aldehyde dehydrogenase | - |
Sphingopyxis macrogoltabida |
PEG-ALDH | - |
Sphingopyxis macrogoltabida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
- |
Sphingopyxis macrogoltabida |
36 | - |
activity assay | Sphingopyxis macrogoltabida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sphingopyxis macrogoltabida |
8 | - |
activity assay | Sphingopyxis macrogoltabida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sphingopyxis macrogoltabida | |
NADP+ | - |
Sphingopyxis macrogoltabida | |
NADP+ | 0.94 mol NADP per subunit | Sphingopyxis macrogoltabida |