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Literature summary for 1.2.1.5 extracted from

  • Ohta, T.; Tani, A.; Kimbara, K.; Kawai, F.
    A novel nicotinoprotein aldehyde dehydrogenase involved in polyethylene glycol degradation (2005), Appl. Microbiol. Biotechnol., 68, 639-646.
    View publication on PubMed

Application

Application Comment Organism
degradation degradation of polyethylene glycols, PEGs Sphingopyxis macrogoltabida

Cloned(Commentary)

Cloned (Comment) Organism
expression as His-tag fusion protein in Echerichia coli Rosetta (DE3) pLys Sphingopyxis macrogoltabida
the pegC gene of Sphingomonas macrogoltabidus is cloned into the pGEM-T and pET23d vectors for expression of nicotinprotein aldehyde dehydrogenase in Escherichia coli Rosetta DE3 pLysS Sphingopyxis macrogoltabida

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 100% inhibition at 1 mM; 1 mM, 100% inhibition Sphingopyxis macrogoltabida
Cu2+ 100% inhibition at 1 mM; 1 mM, 100% inhibition Sphingopyxis macrogoltabida
Hg2+ 100% inhibition at 1 mM; 1 mM, 100% inhibition Sphingopyxis macrogoltabida
p-chloromercuribenzoate 50 microM, 80% inhibition Sphingopyxis macrogoltabida
p-mercuribenzoate 80% inhibition at 0.05 mM Sphingopyxis macrogoltabida
Pb2+ 100% inhibition at 1 mM; 1 mM, 100% inhibition Sphingopyxis macrogoltabida
pyrazole 100% inhibition at 1 mM; 1 mM, 100% inhibition Sphingopyxis macrogoltabida
trans-4-(N,N-dimethylamino)-cinnamaldehyde 1 mM, 58% inhibition; 58% inhibition at 1 mM Sphingopyxis macrogoltabida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.137
-
n-dodecanal 50 mM potassium phosphate buffer pH8.0, 1 mM CaCl2, 36°C Sphingopyxis macrogoltabida

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Sphingopyxis macrogoltabida
Ca2+ stimulating Sphingopyxis macrogoltabida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46100
-
SDS-PAGE, recombinant enzyme Sphingopyxis macrogoltabida
46100
-
molecular weight of one subunit, determined by gel filtration on a Superdex column and by SDS-PAGE Sphingopyxis macrogoltabida
190000
-
190000, molecular mass of the native enzyme is estimated by gel filtration, indicating that the enzyme is a homotetramer Sphingopyxis macrogoltabida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an aldehyde + NAD(P)+ + H2O Sphingopyxis macrogoltabida
-
an acid + NAD(P)H + H+
-
?
an aldehyde + NAD(P)+ + H2O Sphingopyxis macrogoltabida 103
-
an acid + NAD(P)H + H+
-
?

Organism

Organism UniProt Comment Textmining
Sphingopyxis macrogoltabida
-
-
-
Sphingopyxis macrogoltabida 103
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity chromatography Sphingopyxis macrogoltabida
on a Ni-NTA column Sphingopyxis macrogoltabida

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Sphingopyxis macrogoltabida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
cell free extract Sphingopyxis macrogoltabida
0.49
-
after the Ni-NTA purification step Sphingopyxis macrogoltabida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADP+ + H2O
-
Sphingopyxis macrogoltabida acetate + NADPH + H+
-
?
acetaldehyde + NADP+ + H2O
-
Sphingopyxis macrogoltabida 103 acetate + NADPH + H+
-
?
an aldehyde + NAD(P)+ + H2O
-
Sphingopyxis macrogoltabida an acid + NAD(P)H + H+
-
?
an aldehyde + NAD(P)+ + H2O
-
Sphingopyxis macrogoltabida 103 an acid + NAD(P)H + H+
-
?
formaldehyde + NADP+ + H2O
-
Sphingopyxis macrogoltabida formate + NADPH + H+
-
?
formaldehyde + NADP+ + H2O
-
Sphingopyxis macrogoltabida 103 formate + NADPH + H+
-
?
n-butanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-butanoate + NADPH + H+
-
?
n-butanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida 103 n-butanoate + NADPH + H+
-
?
n-decanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-decanoate + NADPH + H+
-
?
n-decanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida 103 n-decanoate + NADPH + H+
-
?
n-dodecanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-dodecanoate + NADPH + H+
-
?
n-hexanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-hexanoate + NADPH + H+
-
?
n-nonanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-nonanoate + NADPH + H+
-
?
n-octanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-octanoate + NADPH + H+
-
?
n-pentanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-pentanoate + NADPH + H+
-
?
n-propanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-propanoate + NADPH + H+
-
?
n-tetradecanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-tetradecanoate + NADPH + H+
-
?
n-tridecanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-tridecanoate + NADPH + H+
-
?
n-undecanal + NADP+ + H2O
-
Sphingopyxis macrogoltabida n-undecanoate + NADPH + H+
-
?
polyethylene glycol 1000-aldehyde + NADP+
-
Sphingopyxis macrogoltabida ? + NADPH
-
?
polyethylene glycol 200-aldehyde + NADP+
-
Sphingopyxis macrogoltabida ? + NADPH
-
?
polyethylene glycol 400-aldehyde + NADP+
-
Sphingopyxis macrogoltabida ? + NADPH
-
?
polyethylene glycol 4000-aldehyde + NADP+ highest activtiy compared to other polyethylene glycolaldehydes Sphingopyxis macrogoltabida ? + NADPH
-
?
polyethylene glycol 6000-aldehyde + NADP+
-
Sphingopyxis macrogoltabida ? + NADPH
-
?

Subunits

Subunits Comment Organism
homotetramer 190000, molecular mass of the native enzyme is estimated by gel filtration, indicating that the enzyme is a homotetramer Sphingopyxis macrogoltabida
homotetramer 4 * 46100, gel filtration Sphingopyxis macrogoltabida

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase
-
Sphingopyxis macrogoltabida
ALDH
-
Sphingopyxis macrogoltabida
nicotinprotein aldehyde dehydrogenase
-
Sphingopyxis macrogoltabida
PEG-ALDH
-
Sphingopyxis macrogoltabida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
-
Sphingopyxis macrogoltabida
36
-
activity assay Sphingopyxis macrogoltabida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sphingopyxis macrogoltabida
8
-
activity assay Sphingopyxis macrogoltabida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sphingopyxis macrogoltabida
NADP+
-
Sphingopyxis macrogoltabida
NADP+ 0.94 mol NADP per subunit Sphingopyxis macrogoltabida