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Literature summary for 1.2.1.46 extracted from

  • Ishibashi, T.; Ikeyama, S.; Amao, Y.
    Activation of the catalytic function of formaldehyde dehydrogenase for formate reduction by single-electron reduced methylviologen (2018), New J. Chem., 42, 18508-18512 .
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0875
-
formate with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + NAD+ + H2O Pseudomonas sp.
-
formate + NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + NAD+ + H2O
-
Pseudomonas sp. formate + NADH + 2 H+
-
?
formate + reduced methyl viologen + 2 H+ single-electron reduced methyl viologen activates the enzyme activity for formate reduction to formaldehyde Pseudomonas sp. formaldehyde + oxidized methyl viologen + H2O
-
?
additional information by using natural co-enzyme NADH, no formaldehyde production due to formate reduction with the enzyme is observed Pseudomonas sp. ?
-
-

Synonyms

Synonyms Comment Organism
FldDH
-
Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.43
-
formate with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.49
-
formate with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication Pseudomonas sp.