KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0875 | - |
formate | with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | Pseudomonas sp. | - |
formate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + NAD+ + H2O | - |
Pseudomonas sp. | formate + NADH + 2 H+ | - |
? | |
formate + reduced methyl viologen + 2 H+ | single-electron reduced methyl viologen activates the enzyme activity for formate reduction to formaldehyde | Pseudomonas sp. | formaldehyde + oxidized methyl viologen + H2O | - |
? | |
additional information | by using natural co-enzyme NADH, no formaldehyde production due to formate reduction with the enzyme is observed | Pseudomonas sp. | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
FldDH | - |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
formate | with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
formate | with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication | Pseudomonas sp. |