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Literature summary for 1.2.1.46 extracted from

  • Ashraf, R.; Rashid, N.; Basheer, S.; Aziz, I.; Akhtar, M.
    Glutathione-dependent formaldehyde dehydrogenase homolog from Bacillus subtilis strain R5 is a propanol-preferring alcohol dehydrogenase (2017), Biochemistry (Moscow), 82, 13-23 .
No PubMed abstract available
Search for more literature for 1.2.1.46

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the purified enzyme on seven types of mesoporous silica (MPS), whose pores are from 2.4 to 31.2 nm sized, avaluatio of the enzyme activity in oxidation of formaldehyde, method overview. Enhancement of catalytic activity is obtained by immobilizing onto MPS, whose pore (mesopore) size is similar to the molecular size of enzyme FDH. FDH immobilized on phenyl-functionalized MPS-4 (MPS-4-Ph) has higher activity than FDH immobilized on non-functionalized one. Immobilized FDH on MPS-4-Ph is active for low formaldehyde concentration form 6.0 M and more sensitive than conventional formaldehyde detectors. The high-order structure of the FDH does not alter upon binding to the non-functionalized MPS surface. But FDH immobilized on functionalized-MPS is changed by hydrophobic interaction or covalent binding Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
SDS 1 mM, over 50% loss of activity for the free enzyme, about 10% loss of activity for the immobilized enzymes Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.438
-
 formaldehyde enzyme immobilized on phenyl-functionalized MPS-4, pH 7.0, 22° Pseudomonas putida
0.926
-
 formaldehyde pH 7.0, 22°C, free enzyme Pseudomonas putida
2.84
-
 formaldehyde enzyme immobilized on MPS-4, pH 7.0, 22° Pseudomonas putida
10.6
-
 formaldehyde enzyme immobilized on methyl-functionalized MPS-4, pH 7.0, 22° Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formaldehyde + NAD+ + H2O Pseudomonas putida
-
 formate + NADH + 2 H+
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol 5%, high loss of activity for the free enzyme, while the immobilzed enzymes are stable Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Pseudomonas putida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + NAD+ + H2O
-
 Pseudomonas putida formate + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.136
-
 formaldehyde enzyme immobilized on MPS-4, pH 7.0, 22° Pseudomonas putida
0.177
-
 formaldehyde enzyme immobilized on phenyl-functionalized MPS-4, pH 7.0, 22° Pseudomonas putida
0.247
-
 formaldehyde enzyme immobilized on methyl-functionalized MPS-4, pH 7.0, 22° Pseudomonas putida
0.48
-
 formaldehyde pH 7.0, 22°C, free enzyme Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.023
-
 formaldehyde enzyme immobilized on methyl-functionalized MPS-4, pH 7.0, 22° Pseudomonas putida
0.047
-
 formaldehyde enzyme immobilized on MPS-4, pH 7.0, 22° Pseudomonas putida
0.404
-
 formaldehyde enzyme immobilized on phenyl-functionalized MPS-4, pH 7.0, 22° Pseudomonas putida
0.518
-
 formaldehyde pH 7.0, 22°C, free enzyme Pseudomonas putida