Literature summary for 1.2.1.46 extracted from

Liao, Y.; Chen, S.; Wang, D.; Zhang, W.; Wang, S.; Ding, J.; Wang, Y.; Cai, L.; Ran, X.; Wang, X.; Zhu, H.
Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa the binary complex with the cofactor NAD+ (2013), Acta Crystallogr. Sect. F, 69, 967-972 .

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the enzyme from Pseudomonas putida is an exception in that it does not depend on glutathione for catalysis	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
gene fdhA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)/pG-TF2	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 15 mg/ml FDH protein and 4 mg/ml NAD+ in 10 mM Tris-HCl, pH 8.0, and 1 mM DTT, with 0.001 ml of reservoir solution containing 0.1 M Bis-Tris, pH 5.5, and 1.8 M ammonium sulfate, at 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement method, and modelling	Pseudomonas aeruginosa

Crystallization (Comment)

Organism

purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 15 mg/ml FDH protein and 4 mg/ml NAD+ in 10 mM Tris-HCl, pH 8.0, and 1 mM DTT, with 0.001 ml of reservoir solution containing 0.1 M Bis-Tris, pH 5.5, and 1.8 M ammonium sulfate, at 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement method, and modelling

Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	required	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
formaldehyde + NAD+ + H2O	Pseudomonas aeruginosa	-	formate + NADH + 2 H+	-	?
formaldehyde + NAD+ + H2O	Pseudomonas aeruginosa LESB58	-	formate + NADH + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HTE3	-	-
Pseudomonas aeruginosa LESB58	Q9HTE3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/pG-TF2 by nickel affinity chromatography dialysis, anion exchange chromatography, and dialysis against crystallization buffer	Pseudomonas aeruginosa

Reaction

Reaction	Comment	Organism	Reaction ID
formaldehyde + NAD+ + H2O = formate + NADH + H+	Pseudomonas aeruginosa FDH has a conserved glutathione-independent ping pong mechanism of formaldehyde oxidization	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formaldehyde + NAD+ + H2O	-	Pseudomonas aeruginosa	formate + NADH + 2 H+	-	?
formaldehyde + NAD+ + H2O	-	Pseudomonas aeruginosa LESB58	formate + NADH + 2 H+	-	?

Subunits

Subunits	Comment	Organism
More	three-dimensional structure modelling and analysis	Pseudomonas aeruginosa
tetramer	the active form with each monomer (350-400 amino-acid residues) consisting of catalytic and coenzyme binding domains together with two bound zinc ions	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
FDH	-	Pseudomonas aeruginosa
fdhA	gene name, UniProt	Pseudomonas aeruginosa
glutathione-independent formaldehyde dehydrogenase	-	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	NAD+-binding mode analysis, with two bound zinc ions per monomer of the tetrameric enzyme	Pseudomonas aeruginosa

General Information

General Information	Comment	Organism
evolution	formaldehyde dehydrogenase (FDH) is a member of the zinc-containing medium-chain alcohol dehydrogenase family	Pseudomonas aeruginosa
physiological function	the enzyme oxidizes toxic formaldehyde to formate using NAD+ as an electron carrier	Pseudomonas aeruginosa