Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | 0.5-2% increases activity in crude extracts | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
native and mutant protein expressed in Escherichia coli DH5alpha | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R197E | very low activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
acetaldehyde | pH 7.5, 25°C | Escherichia coli | |
0.0036 | - |
Chloroacetaldehyde | pH 7.5, 25°C | Escherichia coli | |
0.0058 | - |
propanal | pH 7.5, 25°C | Escherichia coli | |
0.056 | - |
benzaldehyde | pH 7.5, 25°C | Escherichia coli | |
0.1 | - |
NADP+ | pH 7.5, 25°C | Escherichia coli | |
0.9 | - |
4-hydroperoxycyclophosphamide | pH 7.5, 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates activity by about 100% | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56352 | - |
4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry | Escherichia coli |
58000 | - |
4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry | Escherichia coli |
220000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADP+ + H2O | Escherichia coli | - |
acetate + NADPH + H+ | - |
? | |
an aldehyde + NADP+ + H2O | Escherichia coli | removal of aldehydes and alcohols in cells under stress | an acid + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain ATCC 700926D, highest activity in cells grown with ethanol | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
purified enzyme, pH 7.5, 25°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroperoxycyclophosphamide + NADP+ | low activity | Escherichia coli | ? | - |
? | |
acetaldehyde + NADP+ + H2O | - |
Escherichia coli | acetate + NADPH + H+ | - |
? | |
an aldehyde + NADP+ + H2O | removal of aldehydes and alcohols in cells under stress | Escherichia coli | an acid + NADPH + H+ | - |
? | |
benzaldehyde + NADP+ | l40% of the activity with acetaldehyde | Escherichia coli | benzoate + NADPH | - |
? | |
chloroacetaldehyde + NADP+ + H2O | most effective substrate in the absence of Mg2+ | Escherichia coli | chloroacetate + NADPH + H+ | - |
? | |
mafosfamide + NADP+ | low activity | Escherichia coli | ? | - |
? | |
additional information | no activity with glycolaldehyde and glyceraldehyde | Escherichia coli | ? | - |
? | |
propanal + NADP+ + H2O | much lower activity than with acetaldehyde | Escherichia coli | propionate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NAD+ | Escherichia coli | |
NADP+ | - |
Escherichia coli |