Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Gordonia rubripertincta |
expressed in Escherichia coli BL21(DE3) pLysS cells | Rhodococcus opacus |
expressed in Escherichia coli BL21(DE3) pLysS cells | Escherichia coli |
expressed in Escherichia coli BL21(DE3) pLysS cells | Sphingopyxis fribergensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
phenylacetaldehyde | with NAD+ as cosubstrate, at pH 7.7, temperature not specified in the publication | Sphingopyxis fribergensis | |
0.04 | - |
phenylacetaldehyde | with NAD+ as cosubstrate, at pH 7.7, temperature not specified in the publication | Rhodococcus opacus | |
0.06 | - |
phenylacetaldehyde | with NAD+ as cosubstrate, at pH 7.7, temperature not specified in the publication | Gordonia rubripertincta | |
0.08 | - |
NAD+ | with phenylacetaldehyde as cosubstrate, at pH 7.7, temperature not specified in the publication | Gordonia rubripertincta | |
0.38 | - |
NAD+ | with phenylacetaldehyde as cosubstrate, at pH 7.7, temperature not specified in the publication | Rhodococcus opacus | |
0.5 | - |
NAD+ | with phenylacetaldehyde as cosubstrate, at pH 7.7, temperature not specified in the publication | Sphingopyxis fribergensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetaldehyde + NAD+ + H2O | Gordonia rubripertincta | - |
phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | Rhodococcus opacus | - |
phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | Escherichia coli | - |
phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | Sphingopyxis fribergensis | - |
phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | Gordonia rubripertincta CWB2 | - |
phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | Rhodococcus opacus 1CP | - |
phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | Sphingopyxis fribergensis Kp5.2 | - |
phenylacetate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P80668 | - |
- |
Gordonia rubripertincta | - |
- |
- |
Gordonia rubripertincta CWB2 | - |
- |
- |
Rhodococcus opacus | - |
- |
- |
Rhodococcus opacus 1CP | - |
- |
- |
Sphingopyxis fribergensis | A0A0A7PB40 | - |
- |
Sphingopyxis fribergensis Kp5.2 | A0A0A7PB40 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography | Gordonia rubripertincta |
Ni-affinity column chromatography | Rhodococcus opacus |
Ni-affinity column chromatography | Escherichia coli |
Ni-affinity column chromatography | Sphingopyxis fribergensis |
Storage Stability | Organism |
---|---|
-20°C, phosphate buffer (pH 7.7), 99% (v/v) glycerol, 76 days, 44.3% residual activity | Sphingopyxis fribergensis |
-20°C, phosphate buffer (pH 7.7), 99% (v/v) glycerol, 93 days, 92.3% residual activity | Gordonia rubripertincta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methylphenylacetaldehyde + NAD+ + H2O | lowest activity | Gordonia rubripertincta | 1-methylphenylacetate + NADH + 2 H+ | - |
? | |
1-methylphenylacetaldehyde + NAD+ + H2O | lowest activity | Rhodococcus opacus | 1-methylphenylacetate + NADH + 2 H+ | - |
? | |
1-methylphenylacetaldehyde + NAD+ + H2O | lowest activity | Escherichia coli | 1-methylphenylacetate + NADH + 2 H+ | - |
? | |
1-methylphenylacetaldehyde + NAD+ + H2O | lowest activity | Sphingopyxis fribergensis | 1-methylphenylacetate + NADH + 2 H+ | - |
? | |
1-methylphenylacetaldehyde + NAD+ + H2O | lowest activity | Gordonia rubripertincta CWB2 | 1-methylphenylacetate + NADH + 2 H+ | - |
? | |
1-methylphenylacetaldehyde + NAD+ + H2O | lowest activity | Rhodococcus opacus 1CP | 1-methylphenylacetate + NADH + 2 H+ | - |
? | |
1-methylphenylacetaldehyde + NAD+ + H2O | lowest activity | Sphingopyxis fribergensis Kp5.2 | 1-methylphenylacetate + NADH + 2 H+ | - |
? | |
4-chlorophenylacetaldehyde + NAD+ + H2O | - |
Gordonia rubripertincta | 4-chlorophenylacetate + NADH + 2 H+ | - |
? | |
4-chlorophenylacetaldehyde + NAD+ + H2O | - |
Rhodococcus opacus | 4-chlorophenylacetate + NADH + 2 H+ | - |
? | |
4-chlorophenylacetaldehyde + NAD+ + H2O | - |
Escherichia coli | 4-chlorophenylacetate + NADH + 2 H+ | - |
? | |
4-chlorophenylacetaldehyde + NAD+ + H2O | highest activity | Sphingopyxis fribergensis | 4-chlorophenylacetate + NADH + 2 H+ | - |
? | |
4-chlorophenylacetaldehyde + NAD+ + H2O | - |
Gordonia rubripertincta CWB2 | 4-chlorophenylacetate + NADH + 2 H+ | - |
? | |
4-chlorophenylacetaldehyde + NAD+ + H2O | - |
Rhodococcus opacus 1CP | 4-chlorophenylacetate + NADH + 2 H+ | - |
? | |
4-fluorophenylacetaldehyde + NAD+ + H2O | - |
Rhodococcus opacus | 4-fluorophenylacetate + NADH + 2 H+ | - |
? | |
4-fluorophenylacetaldehyde + NAD+ + H2O | - |
Escherichia coli | 4-fluorophenylacetate + NADH + 2 H+ | - |
? | |
4-fluorophenylacetaldehyde + NAD+ + H2O | highest activity | Gordonia rubripertincta | 4-fluorophenylacetate + NADH + 2 H+ | - |
? | |
4-fluorophenylacetaldehyde + NAD+ + H2O | high activity | Sphingopyxis fribergensis | 4-fluorophenylacetate + NADH + 2 H+ | - |
? | |
4-fluorophenylacetaldehyde + NAD+ + H2O | highest activity | Gordonia rubripertincta CWB2 | 4-fluorophenylacetate + NADH + 2 H+ | - |
? | |
additional information | no activity with phenazine methosulfate | Escherichia coli | ? | - |
- |
|
additional information | no activity with phenazine methosulfate | Sphingopyxis fribergensis | ? | - |
- |
|
additional information | no activity with phenazine methosulfate | Sphingopyxis fribergensis Kp5.2 | ? | - |
- |
|
phenylacetaldehyde + NAD+ + H2O | - |
Gordonia rubripertincta | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Rhodococcus opacus | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Escherichia coli | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Sphingopyxis fribergensis | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | highest activity | Rhodococcus opacus | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | highest activity | Escherichia coli | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | highest activity | Sphingopyxis fribergensis | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Gordonia rubripertincta CWB2 | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Rhodococcus opacus 1CP | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | highest activity | Rhodococcus opacus 1CP | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Sphingopyxis fribergensis Kp5.2 | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | highest activity | Sphingopyxis fribergensis Kp5.2 | phenylacetate + NADH + 2 H+ | - |
? | |
phenylacetaldehyde + NADP+ + H2O | 10.6% activity compared to NAD+ | Escherichia coli | phenylacetate + NADPH + 2 H+ | - |
? | |
phenylacetaldehyde + NADP+ + H2O | 29.5% activity compared to NAD+ | Rhodococcus opacus | phenylacetate + NADPH + 2 H+ | - |
? | |
phenylacetaldehyde + NADP+ + H2O | 31.7% activity compared to NAD+ | Gordonia rubripertincta | phenylacetate + NADPH + 2 H+ | - |
? | |
phenylacetaldehyde + NADP+ + H2O | 4% activity compared to NAD+ | Sphingopyxis fribergensis | phenylacetate + NADPH + 2 H+ | - |
? | |
phenylacetaldehyde + NADP+ + H2O | 31.7% activity compared to NAD+ | Gordonia rubripertincta CWB2 | phenylacetate + NADPH + 2 H+ | - |
? | |
phenylacetaldehyde + NADP+ + H2O | 29.5% activity compared to NAD+ | Rhodococcus opacus 1CP | phenylacetate + NADPH + 2 H+ | - |
? | |
phenylacetaldehyde + NADP+ + H2O | 4% activity compared to NAD+ | Sphingopyxis fribergensis Kp5.2 | phenylacetate + NADPH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
feaB | - |
Escherichia coli |
feaB | - |
Sphingopyxis fribergensis |
PAD | - |
Gordonia rubripertincta |
PAD | - |
Rhodococcus opacus |
PAD | - |
Escherichia coli |
PAD | - |
Sphingopyxis fribergensis |
styD | - |
Gordonia rubripertincta |
styD | - |
Rhodococcus opacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | after 30 min at 0-50°C in steps of 5°C, the residual activity after this treatment is compared with the initial activity of the enzyme preparation | Sphingopyxis fribergensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred co-substrate | Gordonia rubripertincta | |
NAD+ | preferred co-substrate | Rhodococcus opacus | |
NAD+ | preferred co-substrate | Escherichia coli | |
NAD+ | preferred co-substrate | Sphingopyxis fribergensis | |
NADP+ | - |
Gordonia rubripertincta | |
NADP+ | - |
Rhodococcus opacus | |
NADP+ | - |
Escherichia coli | |
NADP+ | - |
Sphingopyxis fribergensis |