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Literature summary for 1.2.1.36 extracted from

  • Dominguez, M.; Pequerul, R.; Alvarez, R.; Gimenez-Dejoz, J.; Birta, E.; Porte, S.; Ruehl, R.; Pares, X.; Farres, J.; de Lera, A.
    Synthesis of apocarotenoids by acyclic cross metathesis and characterization as substrates for human retinaldehyde dehydrogenases (2018), Tetrahedron, 74, 2567-2574 .
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
12'-apo-beta-carotenal isoform ALDH1A2, at pH 8.0 and 37°C Homo sapiens
0.003
-
12'-apo-beta-carotenal isoform ALDH1A3, at pH 8.0 and 37°C Homo sapiens
0.005
-
14'-apo-beta-carotenal isoform ALDH1A2, at pH 8.0 and 37°C Homo sapiens
0.012
-
14'-apo-beta-carotenal isoform ALDH1A1, at pH 8.0 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 30 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
retinal + NAD+ + H2O Homo sapiens
-
retinoate + NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O94788
-
-
Homo sapiens P00352
-
-
Homo sapiens P47895
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA chelating Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10'-apo-beta-carotenal + NAD+ + H2O i.e. (2E,4E,6E,8E,10E,12E,14E)-4,9,13-trimethyl-15-(2,6,6-trimethylcyclohex-1-en-1-yl)pentadeca-2,4,6,8,10,12,14-heptaenal Homo sapiens 10'-apo-beta-carotenoic acid + NADH + 2 H+
-
?
12'-apo-beta-carotenal + NAD+ + H2O i.e. (2E,4E,6E,8E,10E,12E)-2,7,11-trimethyl-13-(2,6,6-trimethylcyclohex-1-en-1-yl)trideca-2,4,6,8,10,12-hexaenal Homo sapiens 12'-apo-beta-carotenoic acid + NADH + 2 H+
-
?
14'-apo-beta-carotenal + NAD+ + H2O i.e. (2E,4E,6E,8E,10E)-5,9-dimethyl-11-(2,6,6trimethylcyclohex-1-en-1-yl)undeca-2,4,6,8,10-pentaenal Homo sapiens 14'-apo-beta-carotenoic acid + NADH + 2 H+
-
?
retinal + NAD+ + H2O
-
Homo sapiens retinoate + NADH + 2 H+
-
?

Synonyms

Synonyms Comment Organism
RalDH1 isoform Homo sapiens
RALDH2 isoform Homo sapiens
RALDH3 isoform Homo sapiens
retinaldehyde dehydrogenase
-
Homo sapiens
retinaldehyde dehydrogenase 1
-
Homo sapiens
retinaldehyde dehydrogenase 2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.022
-
12'-apo-beta-carotenal isoform ALDH1A3, at pH 8.0 and 37°C Homo sapiens
0.052
-
14'-apo-beta-carotenal isoform ALDH1A1, at pH 8.0 and 37°C Homo sapiens
0.078
-
14'-apo-beta-carotenal isoform ALDH1A2, at pH 8.0 and 37°C Homo sapiens
0.142
-
12'-apo-beta-carotenal isoform ALDH1A2, at pH 8.0 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme plays a role in the physiological metabolism of apocarotenoids Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.733
-
12'-apo-beta-carotenal isoform ALDH1A1, at pH 8.0 and 37°C Homo sapiens
4.28
-
14'-apo-beta-carotenal isoform ALDH1A1, at pH 8.0 and 37°C Homo sapiens
5.05
-
14'-apo-beta-carotenal isoform ALDH1A3, at pH 8.0 and 37°C Homo sapiens
7.5
-
12'-apo-beta-carotenal isoform ALDH1A3, at pH 8.0 and 37°C Homo sapiens
15.5
-
14'-apo-beta-carotenal isoform ALDH1A2, at pH 8.0 and 37°C Homo sapiens
80
-
12'-apo-beta-carotenal isoform ALDH1A2, at pH 8.0 and 37°C Homo sapiens