Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells as His-tagged enzyme | Mus musculus |
into the vector pET28a for expression in Escherichia coli BL21DE3 cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-ionone | potent inhibitor of isoform RALDH4 activity, at 0.002 mM concentration 9-cis and 13-cis retinal oxidation is reduced by 53% and 63%, respectively | Mus musculus | |
Mg2+ | 4 mM Mg2+ inhibits 9-cis retinal oxidation by isoform RALDH4 by 30% | Mus musculus | |
additional information | beta-ionone has no effect on isoform RALDH3 activity | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
9-cis retinal | - |
Mus musculus | |
0.003 | - |
9-cis retinal | isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C | Mus musculus | |
0.0034 | - |
13-cis retinal | - |
Mus musculus | |
0.0034 | - |
13-cis retinal | isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C | Mus musculus | |
0.0039 | - |
all-trans retinal | - |
Mus musculus | |
0.0039 | - |
all-trans retinal | isoform RALDH3, in 10 mM Tris-HCl buffer, pH 8.5, at 37°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity is slightly activated by magnesium at higher concentrations | Mus musculus | |
Mg2+ | magnesium dramatically activated 13-cis retinal oxidation without significantly affecting 9-cis retinal oxidation | Mus musculus | |
Mg2+ | Mg2+ activates 13-cis retinal oxidation by isoform RALDH4 by 3fold, does not significantly influence 9-cis retinal oxidation, and slightly activates isoform RALDH3 activity, owever, magnesium at lower concentration has no effect on RALDH4 activity | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Mus musculus |
55000 | - |
determined by SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
retinal + NAD+ + H2O | Mus musculus | - |
retinoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus | Q9JHW9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mus musculus |
on a Ni-NTA column | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | adult mouse | Mus musculus | - |
liver | adult mouse | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
13-cis-retinal + NAD+ + H2O | - |
Mus musculus | 13-cis-retinoate + NADH + H+ | - |
? | |
13-cis-retinal + NAD+ + H2O | isoform RALDH4 oxidizes 13-cis retinal with lower catalytic efficiency than 9-cis retinal | Mus musculus | 13-cis-retinoate + NADH + H+ | - |
? | |
9-cis-retinal + NAD+ + H2O | - |
Mus musculus | 9-cis-retinoate + NADH + H+ | - |
? | |
9-cis-retinal + NAD+ + H2O | isoform RALDH4 catalyzes 9-cis retinal oxidation with 3fold higher efficiency than 13-cis retinal | Mus musculus | 9-cis-retinoate + NADH + H+ | - |
? | |
all-trans retinal + NAD+ + H2O | - |
Mus musculus | retinoate + NADH + H+ | - |
? | |
all-trans retinal + NAD+ + H2O | RALDH3 oxidizes all-trans retinal with high catalytic efficiency | Mus musculus | all-trans-retinoate + NADH + H+ | - |
? | |
additional information | show no activity with either 9-cis or 13-cis retinal | Mus musculus | ? | - |
? | |
additional information | isoform RALDH3 does not show activity for either 9-cis or 13-cis retinal substrates, isoform RALDH4 is inactive for all-trans retinal substrate | Mus musculus | ? | - |
? | |
retinal + NAD+ + H2O | - |
Mus musculus | retinoate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RALDH3 | - |
Mus musculus |
RALDH3 | isoform | Mus musculus |
Raldh4 | - |
Mus musculus |
Raldh4 | isoform | Mus musculus |
retinal dehydrogenase 3 | - |
Mus musculus |
retinal dehydrogenase 4 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity assay | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00032 | - |
beta-ionone | isoform RALDH4, using 13-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C | Mus musculus | |
0.00061 | - |
beta-ionone | isoform RALDH4, using 9-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C | Mus musculus |